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GLS2 antibody

Anti-GLS2 antibody used in Western Blot (WB). GTX31828
Anti-GLS2 antibody used in Western Blot (WB). GTX31828
Anti-GLS2 antibody used in Western Blot (WB). GTX31828
Anti-GLS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31828
Anti-GLS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31828
Anti-GLS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31828
Anti-GLS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31828

Cat. No. GTX31828

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all GLS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

Rat Liver Tissue Lysate(GTX27943)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

GLS2 antibody was raised against an 18 amino acid synthetic peptide near the center terminus of human GLS2.The immunogen is located within amino acids 300 - 350 of GLS2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutaminase 2 , GA , GLS , LGA , hLGA

Cellular Localization

Mitochondrion

Background

The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. This protein is functionally similar to the kidney glutaminase but is a little smaller in size. Originally thought to be liver-specific, this protein has been found in other tissues as well. At least one transcribed pseudogene has been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GLS2 antibody used in Western Blot (WB). GTX31828

GTX31828 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GLS2 antibody (GTX31828) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GLS2 antibody used in Western Blot (WB). GTX31828

GTX31828 WB Image

Human tissue extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GLS2 antibody (GTX31828) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GLS2 antibody used in Western Blot (WB). GTX31828

GTX31828 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GLS2 antibody (GTX31828) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GLS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31828

GTX31828 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31828 GLS2 antibody.
Working concentration : 5 μg/ml

Anti-GLS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31828

GTX31828 IHC-P Image

IHC-P analysis of rat liver tissue using GTX31828 GLS2 antibody.
Working concentration : 5 μg/ml

Anti-GLS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31828

GTX31828 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31828 GLS2 antibody.
Working concentration : 20 μg/ml
Green : Primary antibody
Blue : DAPI
Red : Actin

Anti-GLS2 antibody used in IHC (Paraffin sections) (IHC-P). GTX31828

GTX31828 IHC-P Image

IHC-P analysis of rat liver tissue using GTX31828 GLS2 antibody.
Working concentration : 20 μg/ml

REFERENCE

Application Reference

Deguchi-Horiuchi H et al. Sci Rep 2023; 13 (1) : 7291 Pancreatic β-cell glutaminase 2 maintains glucose homeostasis under the condition of hyperglycaemia.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399