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GLUD1+2 antibody

Anti-GLUD1+2 antibody used in Western Blot (WB). GTX112361
Anti-GLUD1+2 antibody used in Western Blot (WB). GTX112361
Anti-GLUD1+2 antibody used in Western Blot (WB). GTX112361
Anti-GLUD1+2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112361
Anti-GLUD1+2 antibody used in IHC (Paraffin sections) (IHC-P). GTX112361

Cat No. GTX112361

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

mouse testis , 293T , A431 , HeLa , HepG2 , rat testis

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GLUD1+2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165504

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

GDH2 antibody , GLUDP1 antibody , GLUD2 antibody , GDH 2 antibody , "glutamate dehydrogenase 2 , mitochondrial antibody" , glutamate dehydrogenase pseudogene 1 antibody , glutamate dehydrogenase 2 antibody

Cellular Localization

Mitochondrion matrix

Background

Glutamate dehydrogenase (EC 1.4.1.3) catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate using NAD and/or NADP as cofactors. See also GLUD1 (MIM 138130).[supplied by OMIM]

Research Area

DATA IMAGES

Anti-GLUD1+2 antibody used in Western Blot (WB). GTX112361

GTX112361 WB Image

Sample (50 μg of whole cell lysate)
A: mouse testis
7.5% SDS PAGE
GTX112361 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GLUD1+2 antibody used in Western Blot (WB). GTX112361

GTX112361 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GLUD1+2 antibody (GTX112361) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GLUD1+2 antibody used in Western Blot (WB). GTX112361

GTX112361 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
7.5% SDS PAGE
GTX112361 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GLUD1+2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112361

GTX112361 ICC/IF Image

GLUD1+2 antibody detects GLUD1+2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GLUD1+2 protein stained by GLUD1+2 antibody (GTX112361) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-GLUD1+2 antibody used in IHC (Paraffin sections) (IHC-P). GTX112361

GTX112361 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using GLUD1+2(GTX112361) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169