GeneTex

  • Country / Location Selection

United States (US)

GLUD1 + GLUD2 antibody

Anti-GLUD1 + GLUD2 antibody used in Western Blot (WB). GTX112361
Anti-GLUD1 + GLUD2 antibody used in Western Blot (WB). GTX112361
Anti-GLUD1 + GLUD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112361
Anti-GLUD1 + GLUD2 antibody used in Western Blot (WB). GTX112361
Anti-GLUD1 + GLUD2 antibody used in Western Blot (WB). GTX112361
Anti-GLUD1 + GLUD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX112361

Cat. No. GTX112361

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all GLUD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

Positive Control

mouse testis , 293T , A431 , HeLa , HepG2 , rat testis

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GLUD1+2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165504

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GDH2 antibody , GLUDP1 antibody , GLUD2 antibody , GDH 2 antibody , "glutamate dehydrogenase 2 , mitochondrial antibody" , glutamate dehydrogenase pseudogene 1 antibody , glutamate dehydrogenase 2 antibody

Cellular Localization

Mitochondrion matrix

Background

Glutamate dehydrogenase (EC 1.4.1.3) catalyzes the reversible oxidative deamination of glutamate to alpha-ketoglutarate using NAD and/or NADP as cofactors. See also GLUD1 (MIM 138130).[supplied by OMIM]

Research Area

DATA IMAGES

Anti-GLUD1 + GLUD2 antibody used in Western Blot (WB). GTX112361

GTX112361 WB Image

Drosophila tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GLUD1 + GLUD2 antibody (GTX112361) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GLUD1 + GLUD2 antibody used in Western Blot (WB). GTX112361

GTX112361 WB Image

Sample (30 μg of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
7.5% SDS PAGE
GTX112361 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GLUD1 + GLUD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112361

GTX112361 ICC/IF Image

GLUD1 + GLUD2 antibody detects GLUD1 + GLUD2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GLUD1 + GLUD2 protein stained by GLUD1 + GLUD2 antibody (GTX112361) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-GLUD1 + GLUD2 antibody used in Western Blot (WB). GTX112361

GTX112361 WB Image

Sample (50 μg of whole cell lysate)
A: mouse testis
7.5% SDS PAGE
GTX112361 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GLUD1 + GLUD2 antibody used in Western Blot (WB). GTX112361

GTX112361 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GLUD1 + GLUD2 antibody (GTX112361) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GLUD1 + GLUD2 antibody used in IHC (Paraffin sections) (IHC-P). GTX112361

GTX112361 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using GLUD1 + GLUD2 antibody (GTX112361) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for GLUD1 + GLUD2 antibody (GTX112361). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for GLUD1 + GLUD2 antibody (GTX112361). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169