GeneTex
United States (US)

GLUT3 antibody

GTX129175_41591_20161013_WB_Fraction_18121410_937.jpg
GTX129175_41591_20161013_WB_Fraction_2_18121410_947.jpg
GTX129175_41591_20171115_ICC_IF_R_18121410_988.jpg
GTX129175_41591_20161020_WB_B_18121410_628.jpg

Cat No. GTX129175

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.

Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

unboiled HepG2 , unboiled HepG2 membrane fraction extract , unboiled U87-MG , unboiled U87-MG membrane fraction extract , GLUT3-transfected 293T
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GLUT3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Solute Carrier Family 2 Member 3,Glut3,Slc2A3

Cellular Localization

Membrane; Multi-pass membrane protein

Database

Research Area

DATA IMAGES
GTX129175_41591_20161013_WB_Fraction_18121410_937.jpg

GTX129175 WB Image

Unboiled U87-MG whole cell and membrane extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GLUT3 antibody (GTX129175) diluted at 1:500. (WCE: whole cell extract; ME: membrane extract)

GTX129175_41591_20161013_WB_Fraction_2_18121410_947.jpg

GTX129175 WB Image

Unboiled HepG2 whole cell and membrane extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GLUT3 antibody (GTX129175) diluted at 1:500. (WCE: whole cell extract; ME: membrane extract)

GTX129175_41591_20171115_ICC_IF_R_18121410_988.jpg

GTX129175 ICC/IF Image

GLUT3 antibody detects GLUT3 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GLUT3 stained by GLUT3 antibody (GTX129175) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX129175_41591_20161020_WB_B_18121410_628.jpg

GTX129175 WB Image

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GLUT3 antibody (GTX129175) diluted at 1:2000.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169