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GLUT4 antibody, C-term

Anti-GLUT4 antibody, C-term used in Western Blot (WB). GTX88031
Anti-GLUT4 antibody, C-term used in Western Blot (WB). GTX88031

Cat. No. GTX88031

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all GLUT4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88031-PEP).

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-TELEYLGPDEND, from the C Terminus of the protein sequence according to NP_001033.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10726449

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 2 member 4 , GLUT4

Cellular Localization

Cell membrane

Background

This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GLUT4 antibody, C-term used in Western Blot (WB). GTX88031

GTX88031 WB Image

GTX88031(1μg/ml) staining of Mouse Heart lysate (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-GLUT4 antibody, C-term used in Western Blot (WB). GTX88031

GTX88031 WB Image

The data was published in the journal PLoS One in 2015.PMID: 26266538

REFERENCE

Application Reference

Ledee D et al. PLoS One 2015; 10 (8) : e0135262 c-Myc Alters Substrate Utilization and O-GlcNAc Protein Posttranslational Modifications without Altering Cardiac Function during Early Aortic Constriction.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399