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GLUT4 antibody, C-term

Anti-GLUT4 antibody, C-term used in Western Blot (WB). GTX88031

Cat No. GTX88031

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.3-1μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88031-PEP).

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS pH7.3, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-TELEYLGPDEND, from the C Terminus of the protein sequence according to NP_001033.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10726449

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

solute carrier family 2 member 4 , GLUT4

Cellular Localization

Cell membrane

Background

This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM). [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GLUT4 antibody, C-term used in Western Blot (WB). GTX88031

GTX88031 WB Image

GTX88031(1μg/ml) staining of Mouse Heart lysate (35μg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

REFERENCE

REVIEW

GLUT4 antibody, C-term Cat No. GTX88031

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015. PMID: 26266538
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Epigenetics Chromatin in 2019. PMID: 31196171
SDS
Sodium Azide.pdf
Package List Price ($)
$ 369