GeneTex
United States (US)

GLYCTK antibody [N3C3]

GTX107807_39911_IHC_19011507_901.jpg
GTX107807_WB_19011507_817.jpg
GTX107807_40317_IFA_19011507_332.jpg

Cat No. GTX107807

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

A431 , HepG2

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.54 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GLYCTK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glycerate Kinase,Hbebp2,Hbebp4,Hbeagbp4A,Glyctk

Cellular Localization

Isoform 1: Cytoplasm , Isoform 2: Cytoplasm , Mitochondrion

Background

This locus encodes a member of the glycerate kinase type-2 family. The encoded enzyme catalyzes the phosphorylation of (R)-glycerate and may be involved in serine degradation and fructose metabolism. Decreased activity of the encoded enzyme may be associated with the disease D-glyceric aciduria. Alternatively spliced transcript variants have been described. [provided by RefSeq]

Database

DATA IMAGES
GTX107807_39911_IHC_19011507_901.jpg

GTX107807 IHC-P Image

Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using GLYCTK(GTX107807) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107807_WB_19011507_817.jpg

GTX107807 WB Image

Sample(30 μg of whole cell lysate)
A:A431(GTX27909)
B:Hep G2(GTX27900)
7.5% SDS PAGE
GTX107807 diluted at 1:500

GTX107807_40317_IFA_19011507_332.jpg

GTX107807 ICC/IF Image

GLYCTK antibody [N3C3] detects GLYCTK protein at cytoplasm by confocal immunofluorescent analysis.
Sample: HepG2 cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min.
Green: GLYCTK protein stained by GLYCTK antibody [N3C3] (GTX107807) diluted at 1:500.
Blue: Hoechst 33342 staining.
[Images captured by Olympus FV10i Confocal Laser Scanning Microscope]

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169