GeneTex
United States (US)

GM-CSF antibody

Cat No. GTX10669

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
Reactivity Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.2 μg/ml
ELISA 0.5-1.0 μg/ml

Note :

ELISA
At 0.5-2 μg/mL the antibody will block 50% of the binding of 5 ng/mL recombinant human HGF to 0.1 μg immobilized recombinant human HGF R/c-MET Fc chimera. >90% of the binding will be blocked by 10 μg/mL of the antibody.

Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Specificity/Sensitivity

By immunoblotting and ELISA, the antibody shows 30% cross-reactivity with recombinant mouse GM-CSF and 10% cross-reactivity with recombinant human GM-CSF.
PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rat

Immunogen

Purified recombinant rat GM-CSF expressed in E. coli.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Four distinct colony-stimulating factors (CSFs) that promote survival, proliferation and differentiation of bone marrow precursor cells are well characterized: granulocyte-macrophage CSF (GMCSF), granulocyte CSF (GCSF), macrophage CSF (MCSF), and interleukin-3 (IL-3, Multi CSF) Both GMCSF and IL-3 are multipotential growth factors, stimulating proliferation of progenitor cells from more than one hematopoietic lineage. In contrast, GCSF and MCSF are lineage restricted hematopoietic growth factors, stimulating the final mitotic divisions and the terminal cellular maturation of partially differentiated hematopoietic progenitors.GMCSF induces myeloid progenitor cells from bone marrow to form colonies containing macrophages and granulocytes in a semisolid media. It acts upon mature macrophages, eosinophils and neutrophils to stimulate various functional activities. GMCSF is an acidic glyocprotein (18-22 kDa in human and 23 kDa inmouse) which binds to high affinity receptors on GMCSF sensitive cells. Although human and mouse GMCSF share 54% amino acid sequence homology, their biological actions are species-specific. The actions of GMCSF and the binding of GMCSF to its receptor are modulated by other growth factors and additional forms of CSF. Human GMCSF is presently undergoing clinical trials for therapy of several diseases and syndromes.

Database

Research Area

SDS
PBS.pdf
Package List Price ($)
$ 319