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GM130 antibody [6D4]

Anti-GM130 antibody [6D4] used in IHC (Paraffin sections) (IHC-P). GTX04699
Anti-GM130 antibody [6D4] used in Flow cytometry (FACS). GTX04699
Anti-GM130 antibody [6D4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04699
Anti-GM130 antibody [6D4] used in IHC (Paraffin sections) (IHC-P). GTX04699
Anti-GM130 antibody [6D4] used in Western Blot (WB). GTX04699
Anti-GM130 antibody [6D4] used in Western Blot (WB). GTX04699
Anti-GM130 antibody [6D4] used in IHC (Paraffin sections) (IHC-P). GTX04699

Cat. No. GTX04699

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6D4

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse, Rat
Package
100 μg ($469)
See all GM130 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.5 μg/mL
ICC/IF 2 μg/mL
IHC-P 0.5-1 μg/mL
FACS 1-3 μg/10⁶ cells
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄

Preservative

0.05 mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E. coli-derived human GM130 recombinant protein (Position: E796-E913).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GM130,GOLGA2,golgin A2

Cellular Localization

Golgi apparatus,Golgi stack membrane

Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-GM130 antibody [6D4] used in IHC (Paraffin sections) (IHC-P). GTX04699

GTX04699 IHC-P Image

IHC-P analysis of human mammary cancer tissue using GTX04699 GM130 antibody [6D4].
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 1 μg/mL

Anti-GM130 antibody [6D4] used in Flow cytometry (FACS). GTX04699

GTX04699 FACS Image

FACS analysis of A431 cells using GTX04699 GM130 antibody [6D4].
Blue : Primary antibody
Green : Isotype control
Red : Unlabeled samples
Dilution : 1 μg/10⁶ cells

Anti-GM130 antibody [6D4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04699

GTX04699 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX04699 GM130 antibody [6D4].
Antigen retrieval : Enzyme antigen retrieval was performed.
Green : Primary antibody
Blue : DAPI
Dilution : 2 μg/mL

Anti-GM130 antibody [6D4] used in IHC (Paraffin sections) (IHC-P). GTX04699

GTX04699 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX04699 GM130 antibody [6D4].
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 1 μg/mL

Anti-GM130 antibody [6D4] used in Western Blot (WB). GTX04699

GTX04699 WB Image

WB analysis of various samples using GTX04699 GM130 antibody [6D4].
Lane 1 : Human placenta tissue lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : Caco-2 whole cell lysate
Lane 5 : A549 whole cell lysate
Lane 6 : A431 whole cell lysate
Lane 7 : HEK293 whole cell lysate
Loading : 50 μg
Dilution : 0.5 μg/mL

Anti-GM130 antibody [6D4] used in Western Blot (WB). GTX04699

GTX04699 WB Image

WB analysis of various samples using GTX04699 GM130 antibody [6D4].
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat liver tissue lysate
Lane 3 : Mouse brain tissue lysate
Lane 4 : Mouse liver tissue lysate
Loading : 50 μg
Dilution : 0.5 μg/mL

Anti-GM130 antibody [6D4] used in IHC (Paraffin sections) (IHC-P). GTX04699

GTX04699 IHC-P Image

IHC-P analysis of rat pancreas tissue using GTX04699 GM130 antibody [6D4].
Antigen retrieval : Heat mediated EDTA buffer (pH 8.0)
Dilution : 1 μg/mL

REFERENCE

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REVIEW

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Package List Price ($)
$ 469