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GM130 antibody

Anti-GM130 antibody used in Immunoprecipitation (IP). GTX54142
Anti-GM130 antibody used in Western Blot (WB). GTX54142
Anti-GM130 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54142

Cat. No. GTX54142

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human GOLGA2 (NP_004477.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

golgin A2 , GM130

Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-GM130 antibody used in Immunoprecipitation (IP). GTX54142

GTX54142 IP Image

IP analysis of HeLa cell lysate using GTX54142 GM130 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-GM130 antibody used in Western Blot (WB). GTX54142

GTX54142 WB Image

WB analysis of various sample lysates using GTX54142 GM130 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-GM130 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54142

GTX54142 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX54142 GM130 antibody.
Blue : DAPI
Dilution : 1:400

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399