GeneTex
United States (US)

GMEB1 antibody

GTX115465_40660_IFA_18121410_441.jpg
GTX115465_40660_IHC_M_18121410_139.jpg
GTX115465_40660_WB_18121410_195.jpg

Cat No. GTX115465

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GMEB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

P96PIF antibody, PIF96 antibody, GMEB1 antibody, glucocorticoid modulatory element-binding protein 1 antibody, DNA-binding protein p96PIF antibody, PIF p96 antibody, parvovirus initiation factor p96 antibody, GMEB-1 antibody, glucocorticoid modulatory element binding protein 1 antibody

Cellular Localization

Nucleus , Cytoplasm

Background

This gene is a member of KDWK gene family. The product of this gene associates with GMEB2 protein, and the complex is essential for parvovirus DNA replication. Study of rat homolog implicates the role of this gene in modulation of transactivation by the glucocorticoid receptor bound to glucocorticoid response elements. Two alternative spliced transcript variants encoding different isoforms exist. [provided by RefSeq]
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Database

DATA IMAGES
GTX115465_40660_IFA_18121410_441.jpg

GTX115465 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GMEB1(GTX115465) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX115465_40660_IHC_M_18121410_139.jpg

GTX115465 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse muscle, using GMEB1(GTX115465) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115465_40660_WB_18121410_195.jpg

GTX115465 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
7.5% SDS PAGE
GTX115465 diluted at 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169