Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (GTX638001) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GNAI1 + GNAI2 + GNAI3 antibody [HL2093] detects GNAI1 + GNAI2 + GNAI3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GNAI1 + GNAI2 + GNAI3 stained by GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (GTX638001) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (GTX638001) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (GTX638001) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI1 + GNAI2 + GNAI3 antibody [HL2093] (GTX638001) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.