APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for human GNAI3 protein, and it does not cross react with human GNAI1 and GNAI2 protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human GNAI3
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
G protein subunit alpha i3 , 87U6 , ARCND1
Cellular Localization
Cytoplasm,Cell membrane,Cytoplasm, cytoskeleton, microtubule organizing center, centrosome,Membrane
Background
Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. [provided by RefSeq, Jun 2012]
Database
Research Area
DATA IMAGES
![Anti-GNAI3 antibody [HL2096] used in Western Blot (WB). GTX638004](/templates/default/js/lightbox/images/loading.gif "Anti-GNAI3 antibody [HL2096] used in Western Blot (WB). GTX638004")
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GTX638004 WB Image
Wild-type (WT) and GNAI3 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI3 antibody [HL2096] (GTX638004) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638004 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI3 antibody [HL2096] (GTX638004) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX638004 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI3 antibody [HL2096] (GTX638004) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638004 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI3 antibody [HL2096] (GTX638004) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638004 IHC-P Image
GNAI3 antibody [HL2096] detects GNAI3 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.GNAI3 stained by GNAI3 antibody [HL2096] (GTX638004) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638004 IHC-P Image
GNAI3 antibody [HL2096] detects GNAI3 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.GNAI3 stained by GNAI3 antibody [HL2096] (GTX638004) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for GNAI3 antibody [HL2096] (GTX638004). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GNAI3 antibody [HL2096] (GTX638004). Be the first to share your experience with this product.
![Anti-GNAI3 antibody [HL2096] used in Western Blot (WB). GTX638004](/upload/website/prouct_img/small/GTX638004/GTX638004_T-44900_20230120_WB_KO_watermark_23013122_138.webp "Anti-GNAI3 antibody [HL2096] used in Western Blot (WB). GTX638004")
![Anti-GNAI3 antibody [HL2096] used in Western Blot (WB). GTX638004](/upload/website/prouct_img/small/GTX638004/GTX638004_T-44900_20230120_WB_M_R_23013122_762.webp "Anti-GNAI3 antibody [HL2096] used in Western Blot (WB). GTX638004")
![Anti-GNAI3 antibody [HL2096] used in Western Blot (WB). GTX638004](/upload/website/prouct_img/small/GTX638004/GTX638004_44977_20230317_WB_23032022_220.webp "Anti-GNAI3 antibody [HL2096] used in Western Blot (WB). GTX638004")
![Anti-GNAI3 antibody [HL2096] used in Western Blot (WB). GTX638004](/upload/website/prouct_img/small/GTX638004/GTX638004_T-44900_20230106_WB_multiple_B_23010922_465.webp "Anti-GNAI3 antibody [HL2096] used in Western Blot (WB). GTX638004")
![Anti-GNAI3 antibody [HL2096] used in IHC (Paraffin sections) (IHC-P). GTX638004](/upload/website/prouct_img/small/GTX638004/GTX638004_T-44900_20230113_IHC-P_multiple_M_23013122_244.webp "Anti-GNAI3 antibody [HL2096] used in IHC (Paraffin sections) (IHC-P). GTX638004")
![Anti-GNAI3 antibody [HL2096] used in IHC (Paraffin sections) (IHC-P). GTX638004](/upload/website/prouct_img/small/GTX638004/GTX638004_T-44900_20230113_IHC-P_multiple_R_23013122_125.webp "Anti-GNAI3 antibody [HL2096] used in IHC (Paraffin sections) (IHC-P). GTX638004")
![Anti-GNAI3 antibody [HL2096] used in Western Blot (WB). GTX638004](/upload/website/prouct_img/normal/GTX638004/GTX638004_T-44900_20230120_WB_KO_watermark_23013122_138.webp "Anti-GNAI3 antibody [HL2096] used in Western Blot (WB). GTX638004")
![Anti-GNAI3 antibody [HL2096] used in Western Blot (WB). GTX638004](/upload/website/prouct_img/normal/GTX638004/GTX638004_T-44900_20230120_WB_M_R_23013122_762.webp "Anti-GNAI3 antibody [HL2096] used in Western Blot (WB). GTX638004")
![Anti-GNAI3 antibody [HL2096] used in Western Blot (WB). GTX638004](/upload/website/prouct_img/normal/GTX638004/GTX638004_44977_20230317_WB_23032022_220.webp "Anti-GNAI3 antibody [HL2096] used in Western Blot (WB). GTX638004")
![Anti-GNAI3 antibody [HL2096] used in Western Blot (WB). GTX638004](/upload/website/prouct_img/normal/GTX638004/GTX638004_T-44900_20230106_WB_multiple_B_23010922_465.webp "Anti-GNAI3 antibody [HL2096] used in Western Blot (WB). GTX638004")
![Anti-GNAI3 antibody [HL2096] used in IHC (Paraffin sections) (IHC-P). GTX638004](/upload/website/prouct_img/normal/GTX638004/GTX638004_T-44900_20230113_IHC-P_multiple_M_23013122_244.webp "Anti-GNAI3 antibody [HL2096] used in IHC (Paraffin sections) (IHC-P). GTX638004")
![Anti-GNAI3 antibody [HL2096] used in IHC (Paraffin sections) (IHC-P). GTX638004](/upload/website/prouct_img/normal/GTX638004/GTX638004_T-44900_20230113_IHC-P_multiple_R_23013122_125.webp "Anti-GNAI3 antibody [HL2096] used in IHC (Paraffin sections) (IHC-P). GTX638004")