Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI3 antibody [HL2096] (GTX638004) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Wild-type (WT) and GNAI3 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI3 antibody [HL2096] (GTX638004) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GNAI3 antibody [HL2096] detects GNAI3 protein by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: GNAI3 stained by GNAI3 antibody [HL2096] (GTX638004) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI3 antibody [HL2096] (GTX638004) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GNAI3 antibody [HL2096] detects GNAI3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
GNAI3 stained by GNAI3 antibody [HL2096] (GTX638004) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GNAI3 antibody [HL2096] detects GNAI3 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
GNAI3 stained by GNAI3 antibody [HL2096] (GTX638004) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI3 antibody [HL2096] (GTX638004) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.