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GNAI3 antibody [N2C3]

KO/KD Orthogonal
Anti-GNAI3 antibody [N2C3] used in Western Blot (WB). GTX103676
Anti-GNAI3 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX103676
Anti-GNAI3 antibody [N2C3] used in Western Blot (WB). GTX103676
Anti-GNAI3 antibody [N2C3] used in Western Blot (WB). GTX103676
Anti-GNAI3 antibody [N2C3] used in Western Blot (WB). GTX103676

Cat. No. GTX103676

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all G protein alpha Inhibitor 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

Mouse brain , A431 , Molt-4 , Raji , HeLa

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Guinea pig, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human G protein alpha Inhibitor 3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950428

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein subunit alpha i3 , 87U6 , ARCND1

Background

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G(k) is the stimulatory G protein of receptor-regulated K(+) channels.

Database

Research Area

DATA IMAGES

Anti-GNAI3 antibody [N2C3] used in Western Blot (WB). GTX103676

GTX103676 WB Image

KO/KD

Wild-type (WT) and GNAI3 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI3 antibody [N2C3] (GTX103676) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GNAI3 antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX103676

GTX103676 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using GNAI3 (GTX103676) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GNAI3 antibody [N2C3] used in Western Blot (WB). GTX103676

GTX103676 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX103676 diluted at 1:1000

Anti-GNAI3 antibody [N2C3] used in Western Blot (WB). GTX103676

GTX103676 WB Image

Sample (30μg whole cell lysate)
A:A431(GTX27909)
B:MOLT4 (GTX27912)
C:Raji (GTX27908)
7.5% SDS PAGE
GTX103676 diluted at 1:1000

Anti-GNAI3 antibody [N2C3] used in Western Blot (WB). GTX103676

GTX103676 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI3 antibody [N2C3] (GTX103676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169