Wild-type (WT) and GNAI3 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI3 antibody [N2C3] (GTX103676) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAI3 antibody [N2C3] (GTX103676) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Sample (30μg whole cell lysate)
A:A431(GTX27909)
B:MOLT4 (GTX27912)
C:Raji (GTX27908)
7.5% SDS PAGE
GTX103676 diluted at 1:1000

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX103676 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using GNAI3 (GTX103676) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min