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GNAI3 antibody

KO/KD
Anti-GNAI3 antibody used in Western Blot (WB). GTX35215
Anti-GNAI3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35215
Anti-GNAI3 antibody used in Western Blot (WB). GTX35215
Anti-GNAI3 antibody used in Immunoprecipitation (IP). GTX35215

Cat. No. GTX35215

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all G protein alpha Inhibitor 3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:100
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-354 of human GNAI3 (NP_006487.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein subunit alpha i3 , 87U6 , ARCND1

Cellular Localization

Cytoplasm,Cell membrane,Membrane

Background

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling pathways. G proteins are composed of 3 units: alpha, beta and gamma. This gene encodes an alpha subunit and belongs to the G-alpha family. Mutation in this gene, resulting in a gly40-to-arg substitution, is associated with auriculocondylar syndrome, and shown to affect downstream targets in the G protein-coupled endothelin receptor pathway. [provided by RefSeq, Jun 2012]

Database

Research Area

DATA IMAGES

Anti-GNAI3 antibody used in Western Blot (WB). GTX35215

GTX35215 WB Image

WB analysis of various sample lysates using GTX35215 GNAI3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-GNAI3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35215

GTX35215 ICC/IF Image

ICC/IF analysis of L929 cells using GTX35215 GNAI3 antibody.
Blue : DAPI
Dilution : 1:100

Anti-GNAI3 antibody used in Western Blot (WB). GTX35215

GTX35215 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35215 GNAI3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-GNAI3 antibody used in Immunoprecipitation (IP). GTX35215

GTX35215 IP Image

IP analysis of MCF-7 cell lysate using GTX35215 GNAI3 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399