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GNAL antibody

Anti-GNAL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103863
Anti-GNAL antibody used in Western Blot (WB). GTX103863
Anti-GNAL antibody used in IHC (Paraffin sections) (IHC-P). GTX103863
Anti-GNAL antibody used in Western Blot (WB). GTX103863

Cat. No. GTX103863

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GNAL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Predict Reactivity

Rat, Zebrafish, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GNAL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950429

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein subunit alpha L , DYT25

Database

Research Area

DATA IMAGES

Anti-GNAL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103863

GTX103863 ICC/IF Image

GNAL antibody detects GNAL protein at cell membrane by immunofluorescent analysis.
Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GNAL stained by GNAL antibody (GTX103863) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GNAL antibody used in Western Blot (WB). GTX103863

GTX103863 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GNAL antibody (GTX103863) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GNAL antibody used in IHC (Paraffin sections) (IHC-P). GTX103863

GTX103863 IHC-P Image

GNAL antibody detects GNAL protein at cytosol on NCI-N87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded NCI-N87 xenograft.
GNAL antibody (GTX103863) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GNAL antibody used in Western Blot (WB). GTX103863

GTX103863 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GNAL antibody (GTX103863) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169