GNAO1 antibody detects GNAO1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: GNAO1 stained by GNAO1 antibody (GTX114439) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GNAO1 antibody detects GNAO1 protein at cell membrane and cytoplasm in rat pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded rat pancreas.
GNAO1 antibody (GTX114439) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GNAO1 antibody detects GNAO1 protein at cell membrane and cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
GNAO1 antibody (GTX114439) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GNAO1 antibody detects GNAO1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GNAO1 stained by GNAO1 antibody (GTX114439) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody (GTX114439) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of GNAO1 protein from HeLa whole cell extracts using 5 μg of GNAO1 antibody (GTX114439).
Western blot analysis was performed using GNAO1 antibody (GTX114439).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody (GTX114439) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAO1 antibody (GTX114439) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GNAO1 antibody detects GNAO1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
GNAO1 antibody (GTX114439) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg of whole cell lysate)
A: adult zebrafish
10% SDS PAGE
GTX114439 diluted at 1:5000

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using GNAO1 antibody (GTX114439) at 1:300 dilution.