GeneTex

  • Country / Location Selection

United States (US)

GNAQ antibody

Anti-GNAQ antibody used in IHC (Paraffin sections) (IHC-P). GTX104544
Anti-GNAQ antibody used in Western Blot (WB). GTX104544
Anti-GNAQ antibody used in IHC (Paraffin sections) (IHC-P). GTX104544
Anti-GNAQ antibody used in Western Blot (WB). GTX104544
Anti-GNAQ antibody used in IHC (Paraffin sections) (IHC-P). GTX104544
Anti-GNAQ antibody used in IHC (Paraffin sections) (IHC-P). GTX104544

Cat. No. GTX104544

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GNAQ products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

3xFlag-human GNAQ-transfected 293T , SK-N-SH

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GNAQ. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036980

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein subunit alpha q , CMC1 , G-ALPHA-q , GAQ , SWS

Background

Guanine nucleotide-binding proteins are a family of heterotrimeric proteins that couple cell surface, 7-transmembrane domain receptors to intracellular signaling pathways. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit. G-alpha-q is the alpha subunit of one of the heterotrimeric GTP-binding proteins that mediates stimulation of phospholipase C-beta (MIM 600230).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-GNAQ antibody used in IHC (Paraffin sections) (IHC-P). GTX104544

GTX104544 IHC-P Image

GNAQ antibody detects GNAQ protein at cell membrane and cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
GNAQ antibody (GTX104544) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GNAQ antibody used in Western Blot (WB). GTX104544

GTX104544 WB Image

GNAQ antibody detects GNAQ protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of 3xFlag-human GNAQ-transfected 293T cells
10 % SDS-PAGE
GNAQ antibody (GTX104544) dilution: 1:1000

Anti-GNAQ antibody used in IHC (Paraffin sections) (IHC-P). GTX104544

GTX104544 IHC-P Image

GNAQ antibody detects GNAQ protein at cytoplasm on human placenta by immunohistochemical analysis.
Sample: Paraffin-embedded placenta.
GNAQ antibody (GTX104544) dilution: 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GNAQ antibody used in Western Blot (WB). GTX104544

GTX104544 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAQ antibody (GTX104544) diluted at 1:500.

Anti-GNAQ antibody used in IHC (Paraffin sections) (IHC-P). GTX104544

GTX104544 IHC-P Image

GNAQ antibody detects GNAQ protein at cytosol on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
GNAQ antibody (GTX104544) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GNAQ antibody used in IHC (Paraffin sections) (IHC-P). GTX104544

GTX104544 IHC-P Image

GNAQ antibody detects GNAQ protein at cytosol on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
GNAQ antibody (GTX104544) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Higa-Nakamine S et al. J Biol Chem 2015; 290 (43) : 25974-85 involvement of protein kinase d1 in signal transduction from the protein kinase c pathway to the tyrosine kinase pathway in response to *-releasing hormone.
Submit a Reference

REVIEW

There are currently no reviews for GNAQ antibody (GTX104544). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169