GeneTex
United States (US)

GNAQ antibody

GTX104544_41038_IHC_M_2_19011506_617.jpg
GTX104544_39904_CT_IHC_19011506_938.jpg
GTX104544_39904_WB_B_19011506_507.jpg
GTX104544_41038_IHC_M_19011506_245.jpg
GTX104544_39904_20160120_IHC-P_R_19011506_475.jpg
GTX104544_39904_20151105_WB_19011506_668.jpg

Cat No. GTX104544

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

3xFlag-human GNAQ-transfected 293T

Predict Reactivity

Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GNAQ. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

G Protein Subunit Alpha Q,Cmc1,G-Alpha-Q,Gaq,Sws,Gnaq

Background

Guanine nucleotide-binding proteins are a family of heterotrimeric proteins that couple cell surface, 7-transmembrane domain receptors to intracellular signaling pathways. Receptor activation catalyzes the exchange of GTP for GDP bound to the inactive G protein alpha subunit resulting in a conformational change and dissociation of the complex. The G protein alpha and beta-gamma subunits are capable of regulating various cellular effectors. Activation is terminated by a GTPase intrinsic to the G-alpha subunit. G-alpha-q is the alpha subunit of one of the heterotrimeric GTP-binding proteins that mediates stimulation of phospholipase C-beta (MIM 600230).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX104544_41038_IHC_M_2_19011506_617.jpg

GTX104544 IHC-P Image

GNAQ antibody detects GNAQ protein at cytosol on mouse ovary by immunohistochemical analysis.
Sample: Paraffin-embedded mouse ovary.
GNAQ antibody (GTX104544) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104544_39904_CT_IHC_19011506_938.jpg

GTX104544 IHC-P Image

GNAQ antibody detects GNAQ protein at cytoplasm on human placenta by immunohistochemical analysis.
Sample: Paraffin-embedded placenta.
GNAQ antibody (GTX104544) dilution: 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104544_39904_WB_B_19011506_507.jpg

GTX104544 WB Image

GNAQ antibody detects GNAQ protein by western blot analysis.
A. 30 μg 293T whole cell extract
B. 30 μg whole cell extract of 3xFlag-human GNAQ-transfected 293T cells
10 % SDS-PAGE
GNAQ antibody (GTX104544) dilution: 1:1000

GTX104544_41038_IHC_M_19011506_245.jpg

GTX104544 IHC-P Image

GNAQ antibody detects GNAQ protein at cytosol on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
GNAQ antibody (GTX104544) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104544_39904_20160120_IHC-P_R_19011506_475.jpg

GTX104544 IHC-P Image

GNAQ antibody detects GNAQ protein at cell membrane and cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
GNAQ antibody (GTX104544) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX104544_39904_20151105_WB_19011506_668.jpg

GTX104544 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAQ antibody (GTX104544) diluted at 1:500.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169