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GNAS antibody [C2C3-2], C-term

Anti-GNAS antibody [C2C3-2], C-term used in Western Blot (WB). GTX113200
Anti-GNAS antibody [C2C3-2], C-term used in Western Blot (WB). GTX113200
Anti-GNAS antibody [C2C3-2], C-term used in Western Blot (WB). GTX113200
Anti-GNAS antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX113200
Anti-GNAS antibody [C2C3-2], C-term used in Western Blot (WB). GTX113200

Cat. No. GTX113200

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Predict Reactivity

Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GNAS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036981

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

GNAS complex locus , AHO , C20orf45 , GNAS1 , GPSA , GSA , GSP , NESP , PITA3 , POH , SCG6 , SgVI

Cellular Localization

Cell membrane,Apical cell membrane,Secreted

Background

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq, Aug 2012]

Database

Research Area

DATA IMAGES

Anti-GNAS antibody [C2C3-2], C-term used in Western Blot (WB). GTX113200

GTX113200 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAS antibody [C2C3-2], C-term (GTX113200) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GNAS antibody [C2C3-2], C-term used in Western Blot (WB). GTX113200

GTX113200 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAS antibody [C2C3-2], C-term (GTX113200) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GNAS antibody [C2C3-2], C-term used in Western Blot (WB). GTX113200

GTX113200 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAS antibody [C2C3-2], C-term (GTX113200) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GNAS antibody [C2C3-2], C-term used in IHC (Paraffin sections) (IHC-P). GTX113200

GTX113200 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using GNAS(GTX113200) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GNAS antibody [C2C3-2], C-term used in Western Blot (WB). GTX113200

GTX113200 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAS antibody [C2C3-2], C-term (GTX113200) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for GNAS antibody [C2C3-2], C-term (GTX113200). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for GNAS antibody [C2C3-2], C-term (GTX113200). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169