GeneTex
United States (US)

GNAS antibody [C2C3], C-term

GTX105877_IHC_19011506_532.jpg
GTX105877_39953_WB_M_brain_19011506_208.jpg
GTX105877_39953_20141024_WB_19011506_376.jpg

Cat No. GTX105877

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 , 46, 68, 111 kDa. ( Note )

Positive Control

A431 , Mouse brain

Predict Reactivity

Rat, Bovine, Dog, Pig, Xenopus laevis, Xenopus tropicalis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GNAS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AHO antibody, C20orf45 antibody, GNAS1 antibody, GPSA antibody, GSA antibody, GSP antibody, MGC33735 antibody, NESP antibody, PHP1A antibody, PHP1B antibody, PHP1C antibody, POH antibody, GNAS antibody, guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 antibody, protein ALEX antibody, guanine nucleotide-binding protein G(s) subunit alpha isoforms XLas antibody, adenylate cyclase-stimulating G alpha protein antibody, neuroendocrine secretory protein antibody, extra large alphas protein antibody, guanine nucleotide regulatory protein antibody, secretogranin VI antibody, alternative gene product encoded by XL-exon antibody, GNAS complex locus antibody

Cellular Localization

Cell membrane; Peripheral membrane protein , Cell projection , ruffle , Cell membrane , Cell projection , ruffle

Background

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contains a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors. [provided by RefSeq]

Research Area

DATA IMAGES
GTX105877_IHC_19011506_532.jpg

GTX105877 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using GNAS(GTX105877) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX105877_39953_WB_M_brain_19011506_208.jpg

GTX105877 WB Image

Sample (50 ug of whole cell lysate)

A: Mouse brain

10% SDS PAGE

GTX105877 diluted at 1:1000

GTX105877_39953_20141024_WB_19011506_376.jpg

GTX105877 WB Image

GNAS antibody detects GNAS protein by western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with GNAS antibody (GTX105877) diluted by 1:500.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169