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GNAT1 antibody [HL2126]

Recombinant antibody Overexpression
Anti-GNAT1 antibody [HL2126] used in Western Blot (WB). GTX638098
Anti-GNAT1 antibody [HL2126] used in Western Blot (WB). GTX638098
Anti-GNAT1 antibody [HL2126] used in Western Blot (WB). GTX638098
Anti-GNAT1 antibody [HL2126] used in Western Blot (WB). GTX638098
Anti-GNAT1 antibody [HL2126] used in IHC (Paraffin sections) (IHC-P). GTX638098
Anti-GNAT1 antibody [HL2126] used in IHC (Paraffin sections) (IHC-P). GTX638098
Anti-GNAT1 antibody [HL2126] used in Western Blot (WB). GTX638098

Cat. No. GTX638098

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2126

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish, Dog
Package
100 μl ($399),
25 μl ($169)
See all GNAT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Product Note

This antibody is specific for human GNAT1 protein, and it does not cross react with human GNAI1, GNAI2, GNAI3, GNAT2, GNAZ, and GNAO1 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human GNAT1

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein subunit alpha transducin 1 , CSNB1G , CSNBAD3 , GBT1 , GNATR

Cellular Localization

Membrane

Background

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES

Anti-GNAT1 antibody [HL2126] used in Western Blot (WB). GTX638098

GTX638098 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [HL2126] (GTX638098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GNAT1 antibody [HL2126] used in Western Blot (WB). GTX638098

GTX638098 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [HL2126] (GTX638098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GNAT1 antibody [HL2126] used in Western Blot (WB). GTX638098

GTX638098 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [HL2126] (GTX638098) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GNAT1 antibody [HL2126] used in Western Blot (WB). GTX638098

GTX638098 WB Image

Human tissue extract (5 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [HL2126] (GTX638098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GNAT1 antibody [HL2126] used in IHC (Paraffin sections) (IHC-P). GTX638098

GTX638098 IHC-P Image

GNAT1 antibody [HL2126] detects GNAT1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat eye.
Green: GNAT1 stained by GNAT1 antibody [HL2126] (GTX638098) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GNAT1 antibody [HL2126] used in IHC (Paraffin sections) (IHC-P). GTX638098

GTX638098 IHC-P Image

GNAT1 antibody [HL2126] detects GNAT1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Green: GNAT1 stained by GNAT1 antibody [HL2126] (GTX638098) diluted at 1:100.
Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GNAT1 antibody [HL2126] used in Western Blot (WB). GTX638098

GTX638098 WB Image

Overexpression

Non-transfected and transfected 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [HL2126] (GTX638098) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations