GeneTex
United States (US)

GNAT1 antibody [N1C3]

GTX105960_40562_20161103_WB_M_eye_19011506_154.jpg
GTX105960_40562_20170214_IHC-Fr_19011506_418.jpg
GTX105960_40562_WB_19011506_719.jpg

Cat No. GTX105960

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-Fr
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

NT2D1 , IMR32 , U87-MG , mouse eye

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Chicken, Guinea pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GNAT1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CSNBAD3 antibody, GBT1 antibody, GNATR antibody, GNAT1 antibody, "transducin, rod-specific antibody", "guanine nucleotide-binding protein G(T), alpha-1 subunit antibody", guanine nucleotide-binding protein G(t) subunit alpha-1 antibody, rod-type transducin alpha subunit antibody, transducin alpha-1 chain antibody, "guanine nucleotide binding protein (G protein), alpha transducing activity polypeptide 1 antibody"

Background

Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX105960_40562_20161103_WB_M_eye_19011506_154.jpg

GTX105960 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [N1C3] (GTX105960) diluted at 1:3000.

GTX105960_40562_20170214_IHC-Fr_19011506_418.jpg

GTX105960 IHC-Fr Image

GNAT1 antibody [N1C3] detects GNAT1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: GNAT1 protein stained by GNAT1 antibody [N1C3] (GTX105960) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

GTX105960_40562_WB_19011506_719.jpg

GTX105960 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
10% SDS PAGE
GTX105960 diluted at 1:3000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169