GeneTex
United States (US)

GNAZ antibody [N2C3]

Comparison
GTX105832_39953_20170209_WB_M_R_competitor_watermark_19011506_270.jpg
GTX105832_39953_20180814_IHC-P_M_19011506_172.jpg
GTX105832_39953_20161229_WB_competitor_watermark_19011506_916.jpg
GTX105832_39953_20161103_WB_M_R_19011506_661.jpg
GTX105832_WB_19011506_483.jpg

Cat No. GTX105832

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Positive Control

HeLa , HepG2 , mouse brain , rat brain

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GNAZ. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GNAZ antibody, guanine nucleotide-binding protein G(z) subunit alpha antibody, g(x) alpha chain antibody, gz-alpha antibody, transducin alpha antibody, "guanine nucleotide binding protein (G protein), alpha z polypeptide antibody"

Cellular Localization

Membrane; Lipid-anchor

Background

The protein encoded by this gene is a member of a G protein subfamily that mediates signal transduction in pertussis toxin-insensitive systms. This encoded protein may play a role in maintaining the ionic balance of perilymphatic and endolymphatic cochlear fluids. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX105832_39953_20170209_WB_M_R_competitor_watermark_19011506_270.jpg

GTX105832 WB Image

Comparison

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with GNAZ antibody [N2C3] (GTX105832) diluted at 1:2000 and competitor's antibody (SC-388) diluted by 1:2000.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX105832_39953_20180814_IHC-P_M_19011506_172.jpg

GTX105832 IHC-P Image

GNAZ antibody [N2C3] detects GNAZ protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GNAZ stained by GNAZ antibody [N2C3] (GTX105832) diluted at 1:500.

GTX105832_39953_20161229_WB_competitor_watermark_19011506_916.jpg

GTX105832 WB Image

Comparison

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membranes were blotted with GNAZ antibody [N2C3] (GTX105832) diluted at 1:500 and competitor's antibody (SC-388) diluted by 1:200.
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX105832_39953_20161103_WB_M_R_19011506_661.jpg

GTX105832 WB Image

Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAZ antibody [N2C3] (GTX105832) diluted at 1:1000.

GTX105832_WB_19011506_483.jpg

GTX105832 WB Image

Sample(30 ug whole cell lysate)
A:Hep G2 (GTX27900)
10% SDS PAGE
GTX105832 diluted at 1:1000

SDS
Glycerol.pdf
Thimerosal.pdf