GeneTex

  • Country / Location Selection

United States (US)

GNB1 antibody

KO/KD
Anti-GNB1 antibody used in IHC (Whole mount) (IHC-Wm). GTX114442
Anti-GNB1 antibody used in Western Blot (WB). GTX114442
Anti-GNB1 antibody used in Western Blot (WB). GTX114442
Anti-GNB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114442
Anti-GNB1 antibody used in Western Blot (WB). GTX114442
Anti-GNB1 antibody used in Immunoprecipitation (IP). GTX114442
Anti-GNB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114442
Anti-GNB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114442
Anti-GNB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114442
Anti-GNB1 antibody used in Western Blot (WB). GTX114442
Anti-GNB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114442
Anti-GNB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114442
Anti-GNB1 antibody used in Western Blot (WB). GTX114442

Cat. No. GTX114442

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm, IP, PLA

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all GNB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm Assay dependent
IP 1:100-1:500
PLA Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

Mouse brain

Predict Reactivity

Bovine, Dog, Chicken, Guinea pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.44 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GNB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10619473

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G protein subunit beta 1 , MRD42

Background

Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. This gene uses alternative polyadenylation signals. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GNB1 antibody used in IHC (Whole mount) (IHC-Wm). GTX114442

GTX114442 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using GNB1 antibody (GTX114442) at 1:200 dilution.

Anti-GNB1 antibody used in Western Blot (WB). GTX114442

GTX114442 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNB1 antibody (GTX114442) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GNB1 antibody used in Western Blot (WB). GTX114442

GTX114442 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNB1 antibody (GTX114442) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GNB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114442

GTX114442 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using GNB1 antibody (GTX114442) at 1:300 dilution.

Anti-GNB1 antibody used in Western Blot (WB). GTX114442

GTX114442 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX114442 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GNB1 antibody used in Immunoprecipitation (IP). GTX114442

GTX114442 IP Image

Immunoprecipitation of GNB1 protein from 293T whole cell extracts using 5 μg of GNB1 antibody (GTX114442).
Western blot analysis was performed using GNB1 antibody (GTX114442).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-GNB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114442

GTX114442 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung papillory adenocarcinoma, using GNB1(GTX114442) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GNB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114442

GTX114442 ICC/IF Image

GNB1 antibody detects GNB1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GNB1 protein stained by GNB1 antibody (GTX114442) diluted at 1:500.
Red: FIS1, a mitochondria marker, stained by FIS1 antibody [GT9810] (GTX631209) diluted at 1:20000.
Blue: Hoechst 33342 staining.
Anti-GNB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114442

GTX114442 IHC-P Image

GNB1 antibody detects GNB1 protein at cell membrane in mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
GNB1 antibody (GTX114442) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GNB1 antibody used in Western Blot (WB). GTX114442

GTX114442 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNB1 antibody (GTX114442) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GNB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX114442

GTX114442 IHC-P Image

GNB1 antibody detects GNB1 protein at cell membrane in rat testis by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
GNB1 antibody (GTX114442) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GNB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114442

GTX114442 ICC/IF Image

The data was published in the journal Front Physiol in 2019. PMID: 31992990

Anti-GNB1 antibody used in Western Blot (WB). GTX114442

GTX114442 WB Image

The data was published in the journal eNeuro in 2018. PMID: 29911170

REFERENCE

Application Reference

Reddy HP et al. iScience 2021; 24 (9) : 103018 Encephalopathy-causing mutations in Gβ1 (GNB1) alter regulation of neuronal GIRK channels.
Greenwood IA et al. Front Physiol 2019; 10 : 1573 The Gβ1 and Gβ3 Subunits Differentially Regulate Rat Vascular Kv7 Channels.
Application : ICC/IF, PLA
Sophie Colombo et al. bioRxiv 2019; (Epub) G protein-coupled potassium channels implicated in mouse and cellular models of GNB1 Encephalopathy
Application : WB, ICC/IF
Reactivity : Mouse
Dexter PM et al. eNeuro 2018; 5 (3) Transducin β-Subunit Can Interact With Multiple G-Protein γ-Subunits to Enable Light Detection by Rod Photoreceptors.
Xu H et al. Dev Biol 2014; 385 (2) : 316-27 Gβ1 controls collective cell migration by regulating the protrusive activity of leader cells in the posterior lateral line primordium.
Application : WB, IHC-Fr
Reactivity : Zebrafish
Show More +
Submit a Reference

REVIEW

There are currently no reviews for GNB1 antibody (GTX114442). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169