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GNMT antibody [8A3]

Overexpression
Anti-GNMT antibody [8A3] used in Flow cytometry (FACS). GTX84425
Anti-GNMT antibody [8A3] used in Western Blot (WB). GTX84425
Anti-GNMT antibody [8A3] used in Western Blot (WB). GTX84425

Cat. No. GTX84425

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8A3

Isotype

 IgG3

Application

 WB, FACS

Reactivity

 Human, Dog, Monkey
Package
100 μl ($519)
See all GNMT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
FACS 1:100
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human GNMT (NP_061833) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycine N-methyltransferase , HEL-S-182mP

Cellular Localization

Cytoplasm

Background

Catalyzes the methylation of glycine by using S-adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine.

Database

Research Area

DATA IMAGES

Anti-GNMT antibody [8A3] used in Flow cytometry (FACS). GTX84425

GTX84425 FACS Image

FACS analysis of HEK293T cells transfected with either GNMT plasmid(Red) or empty vector control plasmid(Blue) using GTX84425 GNMT antibody [8A3].

Anti-GNMT antibody [8A3] used in Western Blot (WB). GTX84425

GTX84425 WB Image

WB analysis of various cell lines using GTX84425 GNMT antibody [8A3].
Loading : 10 ug per lane
Dilution : 1:200

Anti-GNMT antibody [8A3] used in Western Blot (WB). GTX84425

GTX84425 WB Image

Overexpression

WB analysis of HEK293T cells transfected with GNMT plasmid (Right) or empty vector (Left) for 48 hrs using GTX84425 GNMT antibody [8A3].
Loading : 5 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519