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GNMT antibody [AT5D1]

Anti-GNMT antibody [AT5D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57629
Anti-GNMT antibody [AT5D1] used in Western Blot (WB). GTX57629
Anti-GNMT antibody [AT5D1] used in Western Blot (WB). GTX57629

Cat. No. GTX57629

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT5D1

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all GNMT products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT5D1 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GNMT protein

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycine N-methyltransferase , GNMT , HELS182mP , glycine Nmethyltransferase

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along with glycine) to S-adenosyl-L-homocysteine and sarcosine. This protein is found in the cytoplasm and acts as a homotetramer. Defects in this gene are a cause of GNMT deficiency (hypermethioninemia). Alternative splicing results in multiple transcript variants. Naturally occurring readthrough transcription occurs between the upstream CNPY3 (canopy FGF signaling regulator 3) gene and this gene and is represented with GeneID:107080644. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES

Anti-GNMT antibody [AT5D1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57629

GTX57629 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX57629 GNMT antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-GNMT antibody [AT5D1] used in Western Blot (WB). GTX57629

GTX57629 WB Image

WB analysis of various samples using GTX57629 GNMT antibody.
Lane 1 : 293T cell lysate
Lane 2 : GNMT transfected 293T cell lysate
Loading : 10 μg
Dilution : 1:1000

Anti-GNMT antibody [AT5D1] used in Western Blot (WB). GTX57629

GTX57629 WB Image

WB analysis of various samples using GTX57629 GNMT antibody.
Lane 1 : Recombinant Human GNMT protein (50 ng)
Lane 2 : Mouse liver tissue lysate (40 μg)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399