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GNS antibody, Internal

Anti-GNS antibody, Internal used in Flow cytometry (FACS). GTX81833
Anti-GNS antibody, Internal used in Western Blot (WB). GTX81833
Anti-GNS antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81833

Cat. No. GTX81833

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all GNS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
IHC-P 1:50-1:100
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

62 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 283-313 amino acids from the Central region of human GNS.

Purification

Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Conjugation

Unconjugated

RRID

AB_11167918

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glucosamine (N-acetyl)-6-sulfatase , G6S

Cellular Localization

Lysosome

Background

The product of this gene is a lysosomal enzyme found in all cells. It is involved in the catabolism of heparin, heparan sulphate, and keratan sulphate. Deficiency of this enzyme results in the accumulation of undegraded substrate and the lysosomal storage disorder mucopolysaccharidosis type IIID (Sanfilippo D syndrome). Mucopolysaccharidosis type IIID is the least common of the four subtypes of Sanfilippo syndrome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GNS antibody, Internal used in Flow cytometry (FACS). GTX81833

GTX81833 FACS Image

FACS analysis of NCI-H460 cells using GTX81833 GNS antibody, Internal.
Green : primary antibody
Blue : negative control

Anti-GNS antibody, Internal used in Western Blot (WB). GTX81833

GTX81833 WB Image

WB analysis of NCI-H460, HeLa, 293 cell lysate (35ug/lane) using GTX81833 GNS antibody, Internal.

Anti-GNS antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX81833

GTX81833 IHC-P Image

IHC-P analysis of human hepatocarcinoma using GTX81833 GNS antibody, Internal.

REFERENCE

Application Reference

Lai MC et al. PLoS One 2016; 11 (4) : e0153627 Hypoxia Induces Autophagy through Translational Up-Regulation of Lysosomal Proteins in Human Colon Cancer Cells.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399