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GOLGA5 antibody [GT2726]

Anti-GOLGA5 antibody [GT2726] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633810
Anti-GOLGA5 antibody [GT2726] used in Western Blot (WB). GTX633810

Cat. No. GTX633810

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT2726

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GOLGA5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GOLGA5. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888376

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

golgin A5 , GOLIM5 , RFG5 , ret-II

Cellular Localization

Golgi apparatus membrane; Single-pass type IV membrane protein

Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins, whose members localize to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GOLGA5 antibody [GT2726] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633810

GTX633810 ICC/IF Image

GOLGA5 antibody [GT2726] detects GOLGA5 protein at Golgi apparatus by immunofluorescent analysis.
Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GOLGA5 protein stained by GOLGA5 antibody [GT2726] (GTX633810) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-GOLGA5 antibody [GT2726] used in Western Blot (WB). GTX633810

GTX633810 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLGA5 antibody [GT2726] (GTX633810) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations