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GOLGA5 antibody [N2C2], Internal

Anti-GOLGA5 antibody [N2C2], Internal used in Western Blot (WB). GTX104255
Anti-GOLGA5 antibody [N2C2], Internal used in Western Blot (WB). GTX104255
Anti-GOLGA5 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104255
Anti-GOLGA5 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104255
Anti-GOLGA5 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104255
Anti-GOLGA5 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104255

Cat. No. GTX104255

Reference 4
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Hamster
Package
100 μl ($399),
25 μl ($169)
See all GOLGA5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

Mouse brain , A549

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GOLGA5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037117

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

golgin A5 , GOLIM5 , RFG5 , ret-II

Cellular Localization

Golgi apparatus membrane; Single-pass type IV membrane protein

Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins, whose members localize to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GOLGA5 antibody [N2C2], Internal used in Western Blot (WB). GTX104255

GTX104255 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX104255 diluted at 1:1000

Anti-GOLGA5 antibody [N2C2], Internal used in Western Blot (WB). GTX104255

GTX104255 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX104255 diluted at 1:1000

Anti-GOLGA5 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX104255

GTX104255 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human smooth muscle, using GOLGA5(GTX104255) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GOLGA5 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104255

GTX104255 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GOLGA5(GTX104255) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-GOLGA5 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104255

GTX104255 ICC/IF Image

The data was published in the journal Wellcome Open Res in 2019.PMID: 32030357

Anti-GOLGA5 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104255

GTX104255 ICC/IF Image

The data was published in the journal Sci Rep in 2017. PMID: 28615694

REFERENCE

Application Reference

P?rez-Rodriguez S et al. PLoS One 2020; 15 (8) : e0237930 Enrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis.
Application : WB
Reactivity : Hamster
Popa S et al. Wellcome Open Res 2019; 4 : 119 Genome-wide CRISPR screening identifies new regulators of glycoprotein secretion.
Chang HY et al. Sci Rep 2017; 7 (1) : 3515 Selective serotonin reuptake inhibitor, fluoxetine, impairs E-cadherin-mediated cell adhesion and alters calcium homeostasis in pancreatic beta cells.
Application : ICC/IF
Reactivity : Mouse
Reiling JH et al. Nat Cell Biol 2013; 15 (12) : 1473-85 A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

GOLGA5 antibody [N2C2], Internal Cat. No. GTX104255

Rating
( Average 4 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 4 based on 2 users reviews)
Anti-GOLGA5 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104255
Date : Anonymous submitted on 21-Dec-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : RPE cells
Blocking : 0.2% BSA, 0.1% saponin in PBS, 25°C, 0.5Hr
Fixation : 4% paraformaldehyde, 0.3Hr
Primary Antibody : 1:100, 4°C, 16Hr
Anti-GOLGA5 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104255
Date : Anonymous submitted on 25-Nov-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HaCaT cells
Blocking : 2% FBS in PBS, 37°C, 0.5Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.1% Triton X-100, 2% FBS in PBS, 25°C, 30minHr
Primary Antibody : 1:500, 4°C, 16Hr
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169