GeneTex
United States (US)

GOLGA5 antibody [N2C2], Internal

GTX104255_39785_IHC_19011506_274.jpg
GTX104255_39785_WB_M_brain_19011506_106.jpg
GTX104255_WB_19011506_920.jpg
GTX104255_39785_IFA_19011506_380.jpg

Cat No. GTX104255

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

A549 , mouse brain

Predict Reactivity

Rat, Bovine, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GOLGA5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GOLIM5 antibody, RFG5 antibody, ret-II antibody, GOLGA5 antibody, cell proliferation-inducing gene 31 protein antibody, "golgi autoantigen, golgin subfamily a, 5 antibody", golgi integral membrane protein 5 antibody, Golgin subfamily A member 5 antibody, golgin-84 antibody, RET-fused gene 5 protein antibody, golgin A5 antibody

Cellular Localization

Golgi apparatus membrane; Single-pass type IV membrane protein

Background

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins, whose members localize to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues; the chimeric sequences have been designated RET-II and PTC5. [provided by RefSeq]

Database

DATA IMAGES
GTX104255_39785_IHC_19011506_274.jpg

GTX104255 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human smooth muscle, using GOLGA5(GTX104255) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104255_39785_WB_M_brain_19011506_106.jpg

GTX104255 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX104255 diluted at 1:1000

GTX104255_WB_19011506_920.jpg

GTX104255 WB Image

Sample (30 ug of whole cell lysate)
A: A549
7.5% SDS PAGE
GTX104255 diluted at 1:1000

GTX104255_39785_IFA_19011506_380.jpg

GTX104255 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GOLGA5(GTX104255) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

REFERENCE
REVIEW

GOLGA5 antibody [N2C2], Internal Cat No. GTX104255

Rating ( Average 4 based on 2 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 21-Dec-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : RPE cells
Blocking : 0.2% BSA, 0.1% saponin in PBS, 25°C, 0.5Hr
Fixation : 4% paraformaldehyde, 0.3Hr
Primary Antibody : 1:100, 4°C, overnightHr
Date : Anonymous submitted on 25-Nov-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HaCaT cells
Blocking : 2% FBS in PBS, 37°C, 0.5Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.1% Triton X-100, 2% FBS in PBS, 25°C, 30minHr
Primary Antibody : 1:500, 4°C, overnightHr
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169