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GOLPH2 antibody [C3], C-term

KO/KD Orthogonal
Anti-GOLPH2 antibody [C3], C-term used in Western Blot (WB). GTX107702
Anti-GOLPH2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107702
Anti-GOLPH2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107702
Anti-GOLPH2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107702
Anti-GOLPH2 antibody [C3], C-term used in Western Blot (WB). GTX107702
Anti-GOLPH2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107702
Anti-GOLPH2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107702
Anti-GOLPH2 antibody [C3], C-term used in Western Blot (WB). GTX107702
Anti-GOLPH2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107702
Anti-GOLPH2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107702

Cat. No. GTX107702

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GOLPH2 products
PRODUCT

Summary

Golgi membrane protein 1, also known as GP73, GOLM1, and GOLPH2, is a type II Golgi membrane glycoprotein with a predicted molecular weight of 45 kDa. GOLPH2 is often overexpressed in the hepatocytes of patients with hepatitis and hepatocellular carcinoma (HCC). Reports indicate that GOLPH2 is a promising serum marker for the diagnosis of HCC.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:5000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HeLa membrane fraction , DU145 , 293T

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GOLPH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240906

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

golgi membrane protein 1 , C9orf155 , GOLPH2 , GP73 , HEL46 , PSEC0257 , bA379P1.3

Cellular Localization

Golgi apparatus , cis-Golgi network membrane; Single-pass type II membrane protein

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this encoded protein has been observed to be upregulated in response to viral infection. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GOLPH2 antibody [C3], C-term used in Western Blot (WB). GTX107702

GTX107702 WB Image

KO/KD

Wild-type (WT) and GOLPH2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GOLPH2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107702

GTX107702 IHC-P Image

GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
GOLPH2 stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GOLPH2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107702

GTX107702 IHC-P Image

GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
GOLPH2 stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GOLPH2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107702

GTX107702 IHC-P Image

GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
GOLPH2 antibody [C3], C-term (GTX107702) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GOLPH2 antibody [C3], C-term used in Western Blot (WB). GTX107702

GTX107702 WB Image

Orthogonal

GOLPH2 antibody detects GOLPH2 protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa membrane fraction lysate/extract
10 % SDS-PAGE
GOLPH2 antibody (GTX107702) dilution: 1:500

Anti-GOLPH2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107702

GTX107702 ICC/IF Image

Immunofluorescence analysis of 70%methanol,30% Acetone-fixed HCT116 , using GOLPH2(GTX107702) antibody at 1:200 dilution.

Anti-GOLPH2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107702

GTX107702 ICC/IF Image

GOLPH2 antibody [C3], C-term detects GOLPH2 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GOLPH2 protein stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:5000.
Red: Phalloidin, a F-actin marker.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-GOLPH2 antibody [C3], C-term used in Western Blot (WB). GTX107702

GTX107702 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GOLPH2 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107702

GTX107702 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GOLPH2(GTX107702) antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.

Anti-GOLPH2 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107702

GTX107702 IHC-P Image

GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
GOLPH2 stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Truberg J et al. Biochim Biophys Acta Mol Cell Res 2022; 1869 (11) : 119345 Endogenous tagging reveals a mid-Golgi localization of the glycosyltransferase-cleaving intramembrane protease SPPL3
Application : WB
Reactivity : Human
Inoue J et al. J Virol 2019; (Epub) siRNA Screening for the Small GTPase Rab Proteins Identifies that Rab5B is a Major Regulator of Hepatitis B Virus Production.
Application : WB
Reactivity : Human
Ding X et al. Brain Res 2019; 1717 : 117-126 GOLM1 silencing inhibits the proliferation and motility of human glioblastoma cells via the Wnt/β-catenin signaling pathway.
Application : IHC-P
Reactivity : Human
Liu KK et al. Autophagy 2016; 13 (1) : 187-200 Ubiquitin-coated nanodiamonds bind to autophagy receptors for entry into the selective autophagy pathway.
Application : ICC/IF
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169