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GeneTex
United States (US)

GOLPH2 antibody [C3], C-term

KO/KD Orthogonal
GTX107702_41458_20180330_WB_KO_watremark_19011507_747.jpg
GTX107702_41458_20181025_IHC-P_1_19011519_823.jpg
GTX107702_41458_20181025_IHC-P_3_19011519_902.jpg
GTX107702_39715_IHC_19011507_697.jpg
GTX107702_41458_WB_Fraction_19011507_513.jpg
GTX107702_39715_CT_IFA_19011507_618.jpg
GTX107702_41458_IFA_19011507_226.jpg
GTX107702_41458_20170525_WB_19011507_589.jpg
GTX107702_39715_IFA_19011507_439.jpg
GTX107702_41458_20181025_IHC-P_2_19011519_602.jpg

Cat No. GTX107702

Reference 1
Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
See all GOLPH2 products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:5000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , HeLa , HeLa membrane fraction , DU145
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GOLPH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Golgi Membrane Protein 1,C9Orf155,Golph2,Gp73,Hel46,Psec0257,Ba379P1.3,Golm1

Cellular Localization

Golgi apparatus , cis-Golgi network membrane; Single-pass type II membrane protein

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this encoded protein has been observed to be upregulated in response to viral infection. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107702_41458_20180330_WB_KO_watremark_19011507_747.jpg

GTX107702 WB Image

KO/KD

Wild-type (WT) and GOLPH2 knockout (KO) 293T cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX107702_41458_20181025_IHC-P_1_19011519_823.jpg

GTX107702 IHC-P Image

GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
GOLPH2 stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX107702_41458_20181025_IHC-P_3_19011519_902.jpg

GTX107702 IHC-P Image

GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
GOLPH2 stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX107702_39715_IHC_19011507_697.jpg

GTX107702 IHC-P Image

GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
GOLPH2 antibody [C3], C-term (GTX107702) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX107702_41458_WB_Fraction_19011507_513.jpg

GTX107702 WB Image

Orthogonal

GOLPH2 antibody detects GOLPH2 protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa membrane fraction lysate/extract
10 % SDS-PAGE
GOLPH2 antibody (GTX107702) dilution: 1:500
GTX107702_39715_CT_IFA_19011507_618.jpg

GTX107702 ICC/IF Image

Immunofluorescence analysis of 70%methanol,30% Acetone-fixed HCT116 , using GOLPH2(GTX107702) antibody at 1:200 dilution.
GTX107702_41458_IFA_19011507_226.jpg

GTX107702 ICC/IF Image

GOLPH2 antibody [C3], C-term detects GOLPH2 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GOLPH2 protein stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:5000.
Red: Phalloidin, a F-actin marker.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
GTX107702_41458_20170525_WB_19011507_589.jpg

GTX107702 WB Image

Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX107702_39715_IFA_19011507_439.jpg

GTX107702 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GOLPH2(GTX107702) antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.
GTX107702_41458_20181025_IHC-P_2_19011519_602.jpg

GTX107702 IHC-P Image

GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
GOLPH2 stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
REFERENCE

Application Reference

Liu KK et al. Autophagy 2016; 13 (1) : 187-200 Ubiquitin-coated nanodiamonds bind to autophagy receptors for entry into the selective autophagy pathway.
Application : ICC/IF
Reactivity : Human
Submit a Reference
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169