PRODUCT
Summary
Golgi membrane protein 1, also known as GP73, GOLM1, and GOLPH2, is a type II Golgi membrane glycoprotein with a predicted molecular weight of 45 kDa. GOLPH2 is often overexpressed in the hepatocytes of patients with hepatitis and hepatocellular carcinoma (HCC). Reports indicate that GOLPH2 is a promising serum marker for the diagnosis of HCC.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:5000 |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
HeLa membrane fraction , DU145 , 293T
Predict Reactivity
Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human GOLPH2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240906
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
golgi membrane protein 1 , C9orf155 , GOLPH2 , GP73 , HEL46 , PSEC0257 , bA379P1.3
Cellular Localization
Golgi apparatus , cis-Golgi network membrane; Single-pass type II membrane protein
Background
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this encoded protein has been observed to be upregulated in response to viral infection. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX107702 WB Image
Wild-type (WT) and GOLPH2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX107702 IHC-P Image
GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human colon cancer.GOLPH2 stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX107702 IHC-P Image
GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human endometrial carcinoma.GOLPH2 stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX107702 IHC-P Image
GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytosol on human colon carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human colon carcinoma. GOLPH2 antibody [C3], C-term (GTX107702) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX107702 WB Image
GOLPH2 antibody detects GOLPH2 protein by western blot analysis. A. 30 μg HeLa whole cell lysate/extract B. 30 μg HeLa membrane fraction lysate/extract 10 % SDS-PAGE GOLPH2 antibody (GTX107702) dilution: 1:500
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GTX107702 ICC/IF Image
Immunofluorescence analysis of 70%methanol,30% Acetone-fixed HCT116 , using GOLPH2(GTX107702) antibody at 1:200 dilution.
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GTX107702 ICC/IF Image
GOLPH2 antibody [C3], C-term detects GOLPH2 protein at Golgi apparatus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GOLPH2 protein stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:5000. Red: Phalloidin, a F-actin marker. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
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GTX107702 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX107702 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GOLPH2(GTX107702) antibody (Green) at 1:1000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2500.
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GTX107702 IHC-P Image
GOLPH2 antibody [C3], C-term detects GOLPH2 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human ovarian cancer.GOLPH2 stained by GOLPH2 antibody [C3], C-term (GTX107702) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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