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GOLPH2 antibody

Anti-GOLPH2 antibody used in Immunoprecipitation (IP). GTX02179
Anti-GOLPH2 antibody used in Western Blot (WB). GTX02179
Anti-GOLPH2 antibody used in Western Blot (WB). GTX02179
Anti-GOLPH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02179
Anti-GOLPH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX02179
Anti-GOLPH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX02179

Cat No. GTX02179

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:300-1:10000
IHC-P 1:2500-1:10000
IP 1-4 μl/mg of lysate
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2μm-filtered PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Human GOLM1 / GP73 protein

Purification

Purified by Protein A and Antigen Affinity

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

golgi membrane protein 1 , C9orf155 , GOLPH2 , GP73 , HEL46 , PSEC0257 , bA379P1.3

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes proteins synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this gene has been observed to be upregulated in response to viral infection. Alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-GOLPH2 antibody used in Immunoprecipitation (IP). GTX02179

GTX02179 IP Image

IP analysis of HeLa whole cell lysate using GTX02179 GOLPH2 antibody.
IP antibody : 2 μl per 0.5 mg total whole cell lysate
Dilution : 1:200
50 % Protein G agarose : 15 μl

Anti-GOLPH2 antibody used in Western Blot (WB). GTX02179

GTX02179 WB Image

WB analysis of various samples using GTX02179 GOLPH2 antibody.
Lane A : Jurkat whole cell lysate
Lane B : 293T whole cell lysate
Lane C : HeLa whole cell lysate
Lane D : A549 whole cell lysate
Dilution : 1:500
Loading : 30 μg

Anti-GOLPH2 antibody used in Western Blot (WB). GTX02179

GTX02179 WB Image

WB analysis of various samples using GTX02179 GOLPH2 antibody.
Lane A : GOLM1 knockout HeLa whole cell lysate
Lane B : HeLa whole cell lysate
Dilution : 1:500
Loading : 10 μg

Anti-GOLPH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX02179

GTX02179 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX02179 GOLPH2 antibody.
Red : Primary antibody
Blue : DAPI
Dilution : 1:1000

Anti-GOLPH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX02179

GTX02179 IHC-P Image

IHC-P analysis of human hepatoma tissue section using GTX02179 GOLPH2 antibody.
Dilution : 1:5000

Anti-GOLPH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX02179

GTX02179 IHC-P Image

IHC-P analysis of human liver tissue section using GTX02179 GOLPH2 antibody.
Dilution : 1:5000

REFERENCE

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REVIEW

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Package List Price ($)
$ 339