GeneTex
  • Country / Location Selection

United States (US)

GOLPH2 antibody

Anti-GOLPH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116154
Anti-GOLPH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116154
Anti-GOLPH2 antibody used in Western Blot (WB). GTX116154
Anti-GOLPH2 antibody used in Western Blot (WB). GTX116154
Anti-GOLPH2 antibody used in Western Blot (WB). GTX116154
Anti-GOLPH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116154

Cat No. GTX116154

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HeLa , HeLa membrane extract , DU145

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.16 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GOLPH2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171591

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

golgi membrane protein 1 , C9orf155 , GOLPH2 , GP73 , HEL46 , PSEC0257 , bA379P1.3

Cellular Localization

Golgi apparatus , cis-Golgi network membrane; Single-pass type II membrane protein

Background

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of this encoded protein has been observed to be upregulated in response to viral infection. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GOLPH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116154

GTX116154 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using GOLPH2(GTX116154) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-GOLPH2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116154

GTX116154 ICC/IF Image

GOLPH2 antibody detects GOLPH2 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GOLPH2 stained by GOLPH2 antibody (GTX116154) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-GOLPH2 antibody used in Western Blot (WB). GTX116154

GTX116154 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody (GTX116154) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GOLPH2 antibody used in Western Blot (WB). GTX116154

GTX116154 WB Image

HeLa whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody (GTX116154) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GOLPH2 antibody used in Western Blot (WB). GTX116154

GTX116154 WB Image

Wild-type (WT) and GOLPH2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GOLPH2 antibody (GTX116154) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GOLPH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX116154

GTX116154 IHC-P Image

GOLPH2 antibody detects GOLPH2 protein at Golgi apparatus by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
GOLPH2 stained by GOLPH2 antibody (GTX116154) diluted at 1:10000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

GOLPH2 antibody Cat No. GTX116154

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 27-Oct-2015
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : RPE cells
Blocking : 0.2% BSA and 0.1% saponin in PBS, 25°C, 0.5Hr
Fixation : 4% paraformaldehyde, 0.3Hr
Primary Antibody : 1:100, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169