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GOLPH3 antibody

Anti-GOLPH3 antibody used in IHC (Paraffin sections) (IHC-P). GTX85238
Anti-GOLPH3 antibody used in Western Blot (WB). GTX85238

Cat. No. GTX85238

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all GOLPH3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 1 μg/mL
IHC-P 20 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

Rat Lung Tissue Lysate(GTX27941)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

GOLPH3 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human GOLPH3.The immunogen is located within the first 50 amino acids of GOLPH3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

golgi phosphoprotein 3 , GOPP1 , GPP34 , MIDAS , Vps74

Cellular Localization

Cell membrane,Endosome

Background

GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signalling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signalling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.

Database

Research Area

DATA IMAGES

Anti-GOLPH3 antibody used in IHC (Paraffin sections) (IHC-P). GTX85238

GTX85238 IHC-P Image

IHC-P analysis of rat lung tissue using GTX85238 GOLPH3 antibody.
Working concentration : 20 μg/ml

Anti-GOLPH3 antibody used in Western Blot (WB). GTX85238

GTX85238 WB Image

WB analysis of rat lung tissue lysate using GTX85238 GOLPH3 antibody.
Dilution : 0.5μg/ml (Lane A) and 1 μg/ml (Lane B)

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399