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GOT1 (aa 22-35) antibody, Internal

Anti-GOT1 (aa 22-35) antibody, Internal used in Western Blot (WB). GTX88903
Anti-GOT1 (aa 22-35) antibody, Internal used in Western Blot (WB). GTX88903

Cat. No. GTX88903

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all GOT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-1.5μg/ml
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88903-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-TADFREDPDPRKVN, from the internal region of the protein sequence according to NP_002070.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10722153

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamic-oxaloacetic transaminase 1 , AST1 , ASTQTL1 , GIG18 , cAspAT , cCAT

Cellular Localization

Cytoplasm

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-GOT1 (aa 22-35) antibody, Internal used in Western Blot (WB). GTX88903

GTX88903 WB Image

WB analysis of human liver lysate using GTX88903 GOT1 (aa 22-35) antibody, Internal.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

Anti-GOT1 (aa 22-35) antibody, Internal used in Western Blot (WB). GTX88903

GTX88903 WB Image

The data was published in the journal Cancer Metab in 2013. PMID: 24304688

REFERENCE

Application Reference

McGuirk S et al. Cancer Metab 2013; 1 (1) : 22 PGC-1α supports glutamine metabolism in breast cancer.
Application : WB
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399