APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse heart , mouse liver
Predict Reactivity
Rat, Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human GOT1. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888281
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutamic-oxaloacetic transaminase 1 , AST1 , ASTQTL1 , GIG18 , cAspAT , cCAT
Cellular Localization
Cytoplasm
Background
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-GOT1 antibody [GT1255] used in Western Blot (WB). GTX632033](/templates/default/js/lightbox/images/loading.gif "Anti-GOT1 antibody [GT1255] used in Western Blot (WB). GTX632033")
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GTX632033 WB Image
GOT1 antibody detects GOT1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with GOT1 antibody (GTX632033) diluted by 1:1000.
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GTX632033 WB Image
GOT1 antibody detects GOT1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with GOT1 antibody (GTX632033) diluted by 1:1000.
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GTX632033 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT1 antibody [GT1255] (GTX632033) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX632033 WB Image
Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT1 antibody [GT1255] (GTX632033) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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GTX632033 ICC/IF Image
GOT1 antibody [GT1255] detects GOT1 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GOT1 stained by GOT1 antibody [GT1255] (GTX632033) diluted at 1:100.Blue: Hoechst 33342 staining.
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REFERENCE
There are currently no references for GOT1 antibody [GT1255] (GTX632033). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GOT1 antibody [GT1255] (GTX632033). Be the first to share your experience with this product.
![Anti-GOT1 antibody [GT1255] used in Western Blot (WB). GTX632033](/upload/website/prouct_img/small/GTX632033/GTX632033_41925_20141212_WB_M_liver_w_23061202_918.webp "Anti-GOT1 antibody [GT1255] used in Western Blot (WB). GTX632033")
![Anti-GOT1 antibody [GT1255] used in Western Blot (WB). GTX632033](/upload/website/prouct_img/small/GTX632033/GTX632033_41925_20141212_WB_M_heart_w_23061202_822.webp "Anti-GOT1 antibody [GT1255] used in Western Blot (WB). GTX632033")
![Anti-GOT1 antibody [GT1255] used in Western Blot (WB). GTX632033](/upload/website/prouct_img/small/GTX632033/GTX632033_44517_20211126_WB_w_23061202_819.webp "Anti-GOT1 antibody [GT1255] used in Western Blot (WB). GTX632033")
![Anti-GOT1 antibody [GT1255] used in Western Blot (WB). GTX632033](/upload/website/prouct_img/small/GTX632033/GTX632033_41925_20180622_WB_M_tissue_w_23061202_731.webp "Anti-GOT1 antibody [GT1255] used in Western Blot (WB). GTX632033")
![Anti-GOT1 antibody [GT1255] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632033](/upload/website/prouct_img/small/GTX632033/GTX632033_41925_20180801_ICC_IF_w_23061202_133.webp "Anti-GOT1 antibody [GT1255] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632033")
![Anti-GOT1 antibody [GT1255] used in Western Blot (WB). GTX632033](/upload/website/prouct_img/normal/GTX632033/GTX632033_41925_20141212_WB_M_liver_w_23061202_918.webp "Anti-GOT1 antibody [GT1255] used in Western Blot (WB). GTX632033")
![Anti-GOT1 antibody [GT1255] used in Western Blot (WB). GTX632033](/upload/website/prouct_img/normal/GTX632033/GTX632033_41925_20141212_WB_M_heart_w_23061202_822.webp "Anti-GOT1 antibody [GT1255] used in Western Blot (WB). GTX632033")
![Anti-GOT1 antibody [GT1255] used in Western Blot (WB). GTX632033](/upload/website/prouct_img/normal/GTX632033/GTX632033_44517_20211126_WB_w_23061202_819.webp "Anti-GOT1 antibody [GT1255] used in Western Blot (WB). GTX632033")
![Anti-GOT1 antibody [GT1255] used in Western Blot (WB). GTX632033](/upload/website/prouct_img/normal/GTX632033/GTX632033_41925_20180622_WB_M_tissue_w_23061202_731.webp "Anti-GOT1 antibody [GT1255] used in Western Blot (WB). GTX632033")
![Anti-GOT1 antibody [GT1255] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632033](/upload/website/prouct_img/normal/GTX632033/GTX632033_41925_20180801_ICC_IF_w_23061202_133.webp "Anti-GOT1 antibody [GT1255] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632033")