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GOT1 antibody [GT1342]

Anti-GOT1 antibody [GT1342] used in Western Blot (WB). GTX632032
Anti-GOT1 antibody [GT1342] used in Western Blot (WB). GTX632032
Anti-GOT1 antibody [GT1342] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632032

Cat No. GTX632032

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1342

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , mouse liver

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Chicken, Pig, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human GOT1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888280

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glutamic-oxaloacetic transaminase 1 , AST1 , ASTQTL1 , GIG18 , cAspAT , cCAT

Cellular Localization

Cytoplasm

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GOT1 antibody [GT1342] used in Western Blot (WB). GTX632032

GTX632032 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT1 antibody [GT1342] (GTX632032) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GOT1 antibody [GT1342] used in Western Blot (WB). GTX632032

GTX632032 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT1 antibody [GT1342] (GTX632032) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GOT1 antibody [GT1342] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632032

GTX632032 ICC/IF Image

GOT1 antibody [GT1342] detects GOT1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GOT1 stained by GOT1 antibody [GT1342] (GTX632032) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE

There are currently no references for GOT1 antibody [GT1342] (GTX632032). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for GOT1 antibody [GT1342] (GTX632032). Be the first to share your experience with this product.
SDS
PBS.pdf
Package List Price ($)
$ 329
$ 169