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GOT1 antibody [GT1342]

Orthogonal
Anti-GOT1 antibody [GT1342] used in Western Blot (WB). GTX632032
Anti-GOT1 antibody [GT1342] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632032
Anti-GOT1 antibody [GT1342] used in Western Blot (WB). GTX632032

Cat. No. GTX632032

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1342

Isotype

 IgG1

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GOT1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

46 kDa. ( Note )

Positive Control

mouse liver

Predict Reactivity

Rat, Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human GOT1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888280

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamic-oxaloacetic transaminase 1 , AST1 , ASTQTL1 , GIG18 , cAspAT , cCAT

Cellular Localization

Cytoplasm

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GOT1 antibody [GT1342] used in Western Blot (WB). GTX632032

GTX632032 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT1 antibody [GT1342] (GTX632032) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-GOT1 antibody [GT1342] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632032

GTX632032 ICC/IF Image

GOT1 antibody [GT1342] detects GOT1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GOT1 stained by GOT1 antibody [GT1342] (GTX632032) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-GOT1 antibody [GT1342] used in Western Blot (WB). GTX632032

GTX632032 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT1 antibody [GT1342] (GTX632032) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169