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GOT2 antibody [3E9]

Anti-GOT2 antibody [3E9] used in Western Blot (WB). GTX83210
Anti-GOT2 antibody [3E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83210

Cat. No. GTX83210

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3E9

Isotype

 IgG1

Application

 WB, ICC/IF, ELISA

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399)
See all GOT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human GOT2 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamic-oxaloacetic transaminase 2 , KAT4 , KATIV , KYAT4 , mitAAT

Cellular Localization

Mitochondrion matrix,Cell membrane

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]

Database

Research Area

DATA IMAGES

Anti-GOT2 antibody [3E9] used in Western Blot (WB). GTX83210

GTX83210 WB Image

WB analysis of HEK293 (1), PC-12 (2), HL-60 (3), BCBL-1 (4), HepG2 (5) and NIH3T3 (6) cell lysate using GTX83210 GOT2 antibody [3E9].

Anti-GOT2 antibody [3E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83210

GTX83210 ICC/IF Image

ICC/IF analysis of PC-3 (left) and SK-BR-3 (right) cells using GTX83210 GOT2 antibody [3E9].
Green : GOT2
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399