APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Recommended starting dilution is 1:1000. |
| Recommended starting dilution is 1:100. |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Based on internal testing result, this antibody does not cross react with human GOT1.
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT8B11 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human GOT4 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutamic-oxaloacetic transaminase 2 , GOT2 , KAT4 , KATIV , KYAT4 , glutamicoxaloacetic transaminase 2 , mitAAT
Cellular Localization
Mitochondrion matrix,Cell membrane
Background
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]
Database
Research Area
DATA IMAGES
![Anti-GOT2 antibody [AT8B11] used in Western Blot (WB). GTX57618](/templates/default/js/lightbox/images/loading.gif "Anti-GOT2 antibody [AT8B11] used in Western Blot (WB). GTX57618")
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GTX57618 WB Image
WB analysis of various samples using GTX57618 GOT2 antibody.
Lane 1 : Recombinant Human GOT2 protein (50 ng)
Lane 2 : Ramos whole cell lysate (40 μg)
Lane 3 : 293T whole cell lysate (40 μg)
Lane 4 : Liver tissue lysate (40 μg)
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GTX57618 WB Image
WB analysis of various samples using GTX57618 GOT2 antibody.
Lane 1 : Recombinant Human GOT1 protein
Lane 2 : Recombinant Human GOT2 protein
Loading : 20 ng
Dilution : 1:1000
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GTX57618 WB Image
WB analysis of various samples using GTX57618 GOT2 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lane 3 : Balb/3T3 whole cell lysate
Loading : 40 μg
Dilution : 1:1000
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GTX57618 FACS Image
FACS analysis of HepG2 cells using GTX57618 GOT2 antibody.
Cell Number: 1 x 10⁶ cells
Primary antibody: Red line
Antibody amount: 2-5 μg
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GTX57618 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57618 GOT2 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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REFERENCE
There are currently no references for GOT2 antibody [AT8B11] (GTX57618). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GOT2 antibody [AT8B11] (GTX57618). Be the first to share your experience with this product.
![Anti-GOT2 antibody [AT8B11] used in Western Blot (WB). GTX57618](/upload/website/prouct_img/small/GTX57618/GTX57618_20200204_WB1_w_23061123_318.webp "Anti-GOT2 antibody [AT8B11] used in Western Blot (WB). GTX57618")
![Anti-GOT2 antibody [AT8B11] used in Western Blot (WB). GTX57618](/upload/website/prouct_img/small/GTX57618/GTX57618_20200204_WB3_w_23061123_328.webp "Anti-GOT2 antibody [AT8B11] used in Western Blot (WB). GTX57618")
![Anti-GOT2 antibody [AT8B11] used in Western Blot (WB). GTX57618](/upload/website/prouct_img/small/GTX57618/GTX57618_20200204_WB2_w_23061123_284.webp "Anti-GOT2 antibody [AT8B11] used in Western Blot (WB). GTX57618")
![Anti-GOT2 antibody [AT8B11] used in Flow cytometry (FACS). GTX57618](/upload/website/prouct_img/small/GTX57618/GTX57618_20200204_FC_w_23061123_195.webp "Anti-GOT2 antibody [AT8B11] used in Flow cytometry (FACS). GTX57618")
![Anti-GOT2 antibody [AT8B11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57618](/upload/website/prouct_img/small/GTX57618/GTX57618_20200204_IF_w_23061123_421.webp "Anti-GOT2 antibody [AT8B11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57618")
![Anti-GOT2 antibody [AT8B11] used in Western Blot (WB). GTX57618](/upload/website/prouct_img/normal/GTX57618/GTX57618_20200204_WB1_w_23061123_318.webp "Anti-GOT2 antibody [AT8B11] used in Western Blot (WB). GTX57618")
![Anti-GOT2 antibody [AT8B11] used in Western Blot (WB). GTX57618](/upload/website/prouct_img/normal/GTX57618/GTX57618_20200204_WB3_w_23061123_328.webp "Anti-GOT2 antibody [AT8B11] used in Western Blot (WB). GTX57618")
![Anti-GOT2 antibody [AT8B11] used in Western Blot (WB). GTX57618](/upload/website/prouct_img/normal/GTX57618/GTX57618_20200204_WB2_w_23061123_284.webp "Anti-GOT2 antibody [AT8B11] used in Western Blot (WB). GTX57618")
![Anti-GOT2 antibody [AT8B11] used in Flow cytometry (FACS). GTX57618](/upload/website/prouct_img/normal/GTX57618/GTX57618_20200204_FC_w_23061123_195.webp "Anti-GOT2 antibody [AT8B11] used in Flow cytometry (FACS). GTX57618")
![Anti-GOT2 antibody [AT8B11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57618](/upload/website/prouct_img/normal/GTX57618/GTX57618_20200204_IF_w_23061123_421.webp "Anti-GOT2 antibody [AT8B11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57618")