GeneTex

  • Country / Location Selection

United States (US)

GOT2 antibody

KO/KD
Anti-GOT2 antibody used in Western Blot (WB). GTX101929
Anti-GOT2 antibody used in Western Blot (WB). GTX101929
Anti-GOT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101929
Anti-GOT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101929
Anti-GOT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101929
Anti-GOT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101929
Anti-GOT2 antibody used in Western Blot (WB). GTX101929
Anti-GOT2 antibody used in Western Blot (WB). GTX101929
Anti-GOT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101929

Cat. No. GTX101929

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all GOT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

HepG2 , Neuro2A , rat lung , 293T

Predict Reactivity

Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GOT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1240908

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glutamic-oxaloacetic transaminase 2 , KAT4 , KATIV , KYAT4 , mitAAT

Cellular Localization

Mitochondrion matrix , Cell membrane

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GOT2 antibody used in Western Blot (WB). GTX101929

GTX101929 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT2 antibody (GTX101929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GOT2 antibody used in Western Blot (WB). GTX101929

GTX101929 WB Image

GOT2 antibody detects GOT2 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
10% SDS-PAGE
GOT2 antibody (GTX101929) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GOT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101929

GTX101929 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using GOT2(GTX101929) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GOT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101929

GTX101929 IHC-P Image

GOT2 antibody detects GOT2 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
GOT2 antibody (GTX101929) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GOT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101929

GTX101929 IHC-P Image

GOT2 antibody detects GOT2 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma tissue.
GOT2 antibody (GTX101929) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GOT2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101929

GTX101929 IHC-P Image

GOT2 antibody detects GOT2 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GOT2 antibody (GTX101929) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GOT2 antibody used in Western Blot (WB). GTX101929

GTX101929 WB Image

Sample (30 μg of whole cell lysate)
A: HepG2
10% SDS PAGE
GTX101929 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GOT2 antibody used in Western Blot (WB). GTX101929

GTX101929 WB Image

GOT2 antibody detects GOT2 protein by western blot analysis.
A. 50 μg rat lung lysate/extract
10% SDS-PAGE
GOT2 antibody (GTX101929) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GOT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101929

GTX101929 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using GOT2(GTX101929) antibody at 1:500 dilution.

REFERENCE

Application Reference

Arif A et al. Nature 2017; 542 (7641) : 357-361 EPRS is a critical mTORC1-S6K1 effector that influences adiposity in mice.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for GOT2 antibody (GTX101929). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169