GeneTex
United States (US)

GOT2 antibody

GTX101929_40499_20180817_WB_shRNA_watermark_19011506_403.jpg
GTX101929_40499_IHC_R_19011506_738.jpg
GTX101929_40499_IFA_19011506_483.jpg
GTX101929_40499_WB_R_lung_19011506_314.jpg
GTX101929_40499_20150724_IHC-P_M_19011506_946.jpg
GTX101929_40499_WB_M_19011506_315.jpg
GTX101929_40499_IHC_19011506_428.jpg
GTX101929_40499_WB_19011506_809.jpg
GTX101929_40499_20150724_IHC-P_M_2_19011506_733.jpg

Cat No. GTX101929

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

293T , HepG2 , Neuro2A , rat lung

Predict Reactivity

Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GOT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glutamic-Oxaloacetic Transaminase 2,Kat4,Kativ,Kyat4,Mitaat,Got2

Cellular Localization

Mitochondrion matrix , Cell membrane

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101929_40499_20180817_WB_shRNA_watermark_19011506_403.jpg

GTX101929 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT2 antibody (GTX101929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101929_40499_IHC_R_19011506_738.jpg

GTX101929 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using GOT2(GTX101929) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101929_40499_IFA_19011506_483.jpg

GTX101929 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using GOT2(GTX101929) antibody at 1:500 dilution.

GTX101929_40499_WB_R_lung_19011506_314.jpg

GTX101929 WB Image

GOT2 antibody detects GOT2 protein by western blot analysis.
A. 50 µg rat lung lysate/extract
10% SDS-PAGE
GOT2 antibody (GTX101929) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101929_40499_20150724_IHC-P_M_19011506_946.jpg

GTX101929 IHC-P Image

GOT2 antibody detects GOT2 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
GOT2 antibody (GTX101929) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101929_40499_WB_M_19011506_315.jpg

GTX101929 WB Image

GOT2 antibody detects GOT2 protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
10% SDS-PAGE
GOT2 antibody (GTX101929) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101929_40499_IHC_19011506_428.jpg

GTX101929 IHC-P Image

GOT2 antibody detects GOT2 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma tissue.
GOT2 antibody (GTX101929) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101929_40499_WB_19011506_809.jpg

GTX101929 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2
10% SDS PAGE
GTX101929 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101929_40499_20150724_IHC-P_M_2_19011506_733.jpg

GTX101929 IHC-P Image

GOT2 antibody detects GOT2 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GOT2 antibody (GTX101929) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169