GeneTex
United States (US)

GOT2 antibody

KO/KD
GTX101929_40499_20180817_WB_shRNA_watermark_19011506_403.jpg
GTX101929_40499_IHC_R_19011506_738.jpg
GTX101929_40499_IFA_19011506_483.jpg
GTX101929_40499_WB_R_lung_19011506_314.jpg
GTX101929_40499_20150724_IHC-P_M_19011506_946.jpg
GTX101929_40499_WB_M_19011506_315.jpg
GTX101929_40499_IHC_19011506_428.jpg
GTX101929_40499_WB_19011506_809.jpg
GTX101929_40499_20150724_IHC-P_M_2_19011506_733.jpg

Cat No. GTX101929

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

293T , HepG2 , Neuro2A , rat lung

Predict Reactivity

Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GOT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ40994 antibody, KAT4 antibody, KATIV antibody, mitAAT antibody, GOT2 antibody, aspartate aminotransferase, mitochondrial antibody, fatty acid-binding protein antibody, FABPpm antibody, glutamate oxaloacetate transaminase 2 antibody, kynurenine aminotransferase IV antibody, transaminase A antibody, mAspAT antibody, plasma membrane-associated fatty acid-binding protein antibody, FABP-1 antibody, glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) antibody

Cellular Localization

Mitochondrion matrix , Cell membrane

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101929_40499_20180817_WB_shRNA_watermark_19011506_403.jpg

GTX101929 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT2 antibody (GTX101929) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101929_40499_IHC_R_19011506_738.jpg

GTX101929 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using GOT2(GTX101929) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101929_40499_IFA_19011506_483.jpg

GTX101929 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using GOT2(GTX101929) antibody at 1:500 dilution.

GTX101929_40499_WB_R_lung_19011506_314.jpg

GTX101929 WB Image

GOT2 antibody detects GOT2 protein by western blot analysis.
A. 50 µg rat lung lysate/extract
10% SDS-PAGE
GOT2 antibody (GTX101929) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101929_40499_20150724_IHC-P_M_19011506_946.jpg

GTX101929 IHC-P Image

GOT2 antibody detects GOT2 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
GOT2 antibody (GTX101929) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101929_40499_WB_M_19011506_315.jpg

GTX101929 WB Image

GOT2 antibody detects GOT2 protein by western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
10% SDS-PAGE
GOT2 antibody (GTX101929) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101929_40499_IHC_19011506_428.jpg

GTX101929 IHC-P Image

GOT2 antibody detects GOT2 protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma tissue.
GOT2 antibody (GTX101929) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101929_40499_WB_19011506_809.jpg

GTX101929 WB Image

Sample (30 µg of whole cell lysate)
A: HepG2
10% SDS PAGE
GTX101929 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101929_40499_20150724_IHC-P_M_2_19011506_733.jpg

GTX101929 IHC-P Image

GOT2 antibody detects GOT2 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GOT2 antibody (GTX101929) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169