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GOT2 antibody

Anti-GOT2 antibody used in Western Blot (WB). GTX101930
Anti-GOT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101930
Anti-GOT2 antibody used in Western Blot (WB). GTX101930
Anti-GOT2 antibody used in Western Blot (WB). GTX101930
Anti-GOT2 antibody used in Western Blot (WB). GTX101930

Cat No. GTX101930

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , A375 , NIH-3T3 , JC , BCL-1 , C2C12 , Raw264.7

Predict Reactivity

Rat, Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GOT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glutamic-oxaloacetic transaminase 2 , KAT4 , KATIV , KYAT4 , mitAAT

Cellular Localization

Mitochondrion matrix , Cell membrane

Background

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GOT2 antibody used in Western Blot (WB). GTX101930

GTX101930 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT2 antibody (GTX101930) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GOT2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101930

GTX101930 ICC/IF Image

GOT2 antibody detects GOT2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GOT2 stained by GOT2 antibody (GTX101930) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-GOT2 antibody used in Western Blot (WB). GTX101930

GTX101930 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
D: C2C12
E: Raw264.7
10% SDS PAGE
GTX101930 diluted at 1:10000

Anti-GOT2 antibody used in Western Blot (WB). GTX101930

GTX101930 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT2 antibody (GTX101930) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-GOT2 antibody used in Western Blot (WB). GTX101930

GTX101930 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT2 antibody (GTX101930) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169