APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
NIH-3T3 , JC , BCL-1 , C2C12 , Raw264.7 , 293T
Predict Reactivity
Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.33 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human GOT2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240909
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glutamic-oxaloacetic transaminase 2 , KAT4 , KATIV , KYAT4 , mitAAT
Cellular Localization
Mitochondrion matrix , Cell membrane
Background
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX101930 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT2 antibody (GTX101930) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101930 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
D: C2C12
E: Raw264.7
10% SDS PAGE
GTX101930 diluted at 1:10000
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GTX101930 ICC/IF Image
GOT2 antibody detects GOT2 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: GOT2 stained by GOT2 antibody (GTX101930) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 μm.
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GTX101930 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT2 antibody (GTX101930) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101930 IHC-P Image
GOT2 antibody detects GOT2 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.GOT2 stained by GOT2 antibody (GTX101930) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101930 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT2 antibody (GTX101930) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX101930 IHC-P Image
GOT2 antibody detects GOT2 protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.GOT2 stained by GOT2 antibody (GTX101930) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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