Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT2 antibody (GTX101930) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT2 antibody (GTX101930) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GOT2 antibody (GTX101930) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GOT2 antibody detects GOT2 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
GOT2 stained by GOT2 antibody (GTX101930) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GOT2 antibody detects GOT2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
GOT2 stained by GOT2 antibody (GTX101930) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GOT2 antibody detects GOT2 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: GOT2 stained by GOT2 antibody (GTX101930) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
D: C2C12
E: Raw264.7
10% SDS PAGE
GTX101930 diluted at 1:10000