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GPAM antibody

Anti-GPAM antibody used in IHC (Paraffin sections) (IHC-P). GTX85034
Anti-GPAM antibody used in IHC (Paraffin sections) (IHC-P). GTX85034
Anti-GPAM antibody used in Western Blot (WB). GTX85034
Anti-GPAM antibody used in IHC (Paraffin sections) (IHC-P). GTX85034
Anti-GPAM antibody used in IHC (Paraffin sections) (IHC-P). GTX85034

Cat. No. GTX85034

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all GPAM products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 2.5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

94 kDa. ( Note )

Positive Control

Rat Brain Tissue Lysate(GTX27942)

Product Note

At least four isoforms are known to exist; this antibody will detect only the two largest isoforms.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

GPAM antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of the human GPAM .The immunogen is located within amino acids 730 - 780 of GPAM.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycerol-3-phosphate acyltransferase, mitochondrial , GPAT , GPAT1

Cellular Localization

Mitochondrion outer membrane

Background

Glycerol-3-phosphate acyltransferase 1 (GPAT1), one of four known GPAT isoforms, is located on the mitochondrial outer membrane, allowing reciprocal regulation with carnitine palmitoyltransferase-1. It is thought to be critical for the development of hepatic steatosis; steatosis triggered by GPAT1 overexpression leads to hepatic and possibly peripheral insulin resistance. GPAT1 is transcriptionally upregulated by insulin and sterol regulatory element binding protein (SREBP-1) and downregulated by AMP-activated protein kinase. Mice deficient in GPAT1 exhibit decreased triacylglycerol (TAG) in cardiomyocytes even in high-fat diets, suggesting that GPAT1 contributes significantly to TAG accumulation in heart tissue during lipogenic or high fat diets. At least two isoforms of GPAT1 are known to exist.

Database

Research Area

DATA IMAGES

Anti-GPAM antibody used in IHC (Paraffin sections) (IHC-P). GTX85034

GTX85034 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85034 GPAM antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 20 μg/ml

Anti-GPAM antibody used in IHC (Paraffin sections) (IHC-P). GTX85034

GTX85034 IHC-P Image

IHC-P analysis of rat brain tissue using GTX85034 GPAM antibody.
Working concentration : 2.5 μg/ml

Anti-GPAM antibody used in Western Blot (WB). GTX85034

GTX85034 WB Image

WB analysis of rat brain tissue lysate using GTX85034 GPAM antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-GPAM antibody used in IHC (Paraffin sections) (IHC-P). GTX85034

GTX85034 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX85034 GPAM antibody.
Working concentration : 5 μg/ml

Anti-GPAM antibody used in IHC (Paraffin sections) (IHC-P). GTX85034

GTX85034 IHC-P Image

IHC-P analysis of rat brain tissue using GTX85034 GPAM antibody.
Working concentration : 20 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399