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GPBB antibody

Orthogonal
Anti-GPBB antibody used in IHC (Paraffin sections) (IHC-P). GTX104291
Anti-GPBB antibody used in IHC (Paraffin sections) (IHC-P). GTX104291
Anti-GPBB antibody used in Western Blot (WB). GTX104291
Anti-GPBB antibody used in Western Blot (WB). GTX104291
Anti-GPBB antibody used in IHC (Paraffin sections) (IHC-P). GTX104291
Anti-GPBB antibody used in Western Blot (WB). GTX104291

Cat. No. GTX104291

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all GPBB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , HeLa , HepG2

Predict Reactivity

Rat, Zebrafish, Sheep, Bovine, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.36 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GPBB. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951560

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycogen phosphorylase B , GPBB

Background

The protein encoded by this gene is a glycogen phosphorylase found predominantly in the brain. The encoded protein forms homodimers which can associate into homotetramers, the enzymatically active form of glycogen phosphorylase. The activity of this enzyme is positively regulated by AMP and negatively regulated by ATP, ADP, and glucose-6-phosphate. This enzyme catalyzes the rate-determining step in glycogen degradation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-GPBB antibody used in IHC (Paraffin sections) (IHC-P). GTX104291

GTX104291 IHC-P Image

GPBB antibody detects GPBB protein at cytosol on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
GPBB antibody (GTX104291) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GPBB antibody used in IHC (Paraffin sections) (IHC-P). GTX104291

GTX104291 IHC-P Image

GPBB antibody detects PYGB protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
GPBB antibody (GTX104291) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GPBB antibody used in Western Blot (WB). GTX104291

GTX104291 WB Image

Sample (50 μg of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
GTX104291 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GPBB antibody used in Western Blot (WB). GTX104291

GTX104291 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GPBB antibody (GTX104291) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GPBB antibody used in IHC (Paraffin sections) (IHC-P). GTX104291

GTX104291 IHC-P Image

Orthogonal

The data was published in the journal Sci Rep in 2018. PMID: 29555943

Anti-GPBB antibody used in Western Blot (WB). GTX104291

GTX104291 WB Image

The data was published in the journal Sci Rep in 2018. PMID: 29555943

REFERENCE

Application Reference

Cooper ML et al. Proc Natl Acad Sci U S A 2020; 117 (31) : 18810-18821 Redistribution of metabolic resources through astrocyte networks mitigates neurodegenerative stress.
Geiszler PC et al. Sci Rep 2018; 8 (1) : 4833 Dynamic metabolic patterns tracking neurodegeneration and gliosis following 26S proteasome dysfunction in mouse forebrain neurons.
Application : IHC-P
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169