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GPD1 antibody [HL1959]

Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796
Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796
Anti-GPD1 antibody [HL1959] used in IHC (Paraffin sections) (IHC-P). GTX637796
Anti-GPD1 antibody [HL1959] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637796
Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796

Cat. No. GTX637796

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1959

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Product Note

This antibody is specific for human GPD1 protein, and it does not cross-react with human GPDL1 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-term region of human GPD1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycerol-3-phosphate dehydrogenase 1 , GPD-C , GPDH-C , HTGTI

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the NAD-dependent glycerol-3-phosphate dehydrogenase family. The encoded protein plays a critical role in carbohydrate and lipid metabolism by catalyzing the reversible conversion of dihydroxyacetone phosphate (DHAP) and reduced nicotine adenine dinucleotide (NADH) to glycerol-3-phosphate (G3P) and NAD+. The encoded cytosolic protein and mitochondrial glycerol-3-phosphate dehydrogenase also form a glycerol phosphate shuttle that facilitates the transfer of reducing equivalents from the cytosol to mitochondria. Mutations in this gene are a cause of transient infantile hypertriglyceridemia. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]

Database

Research Area

DATA IMAGES

Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796

GTX637796 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPD1 antibody [HL1959] (GTX637796) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796

GTX637796 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPD1 antibody [HL1959] (GTX637796) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-GPD1 antibody [HL1959] used in IHC (Paraffin sections) (IHC-P). GTX637796

GTX637796 IHC-P Image

GPD1 antibody [HL1959] detects GPD1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
GPD1 stained by GPD1 antibody [HL1959] (GTX637796) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPD1 antibody [HL1959] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637796

GTX637796 ICC/IF Image

GPD1 antibody [HL1959] detects GPD1 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: Caco-2 cells were fixed in ice-cold MeOH for 5 min.
Green: GPD1 stained by GPD1 antibody [HL1959] (GTX637796) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-GPD1 antibody [HL1959] used in Western Blot (WB). GTX637796

GTX637796 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPD1 antibody [HL1959] (GTX637796) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations