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GPD1 antibody

Anti-GPD1 antibody used in Western Blot (WB). GTX103948
Anti-GPD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103948
Anti-GPD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103948
Anti-GPD1 antibody used in Western Blot (WB). GTX103948

Cat No. GTX103948

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

HepG2 , mouse brain

Predict Reactivity

Rat, Bovine, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.74 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GPD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

glycerol-3-phosphate dehydrogenase 1 , GPD-C , GPDH-C , HTGTI

Cellular Localization

Cytoplasm

Database

DATA IMAGES
Anti-GPD1 antibody used in Western Blot (WB). GTX103948

GTX103948 WB Image

Sample (50 µg of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX103948 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-GPD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX103948

GTX103948 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human stomach , using GPD1(GTX103948) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GPD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103948

GTX103948 ICC/IF Image

GPD1 antibody detects GPD1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GPD1 protein stained by GPD1 antibody (GTX103948) diluted at 1:500.
Blue: Hoechst 33343 staining.

Anti-GPD1 antibody used in Western Blot (WB). GTX103948

GTX103948 WB Image

Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with GPD1 antibody (GTX103948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169