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GPD1 antibody

Anti-GPD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117910
Anti-GPD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117910
Anti-GPD1 antibody used in Western Blot (WB). GTX117910
Anti-GPD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117910
Anti-GPD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117910

Cat. No. GTX117910

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all GPD1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Mouse, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.51 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GPD1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10732547

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycerol-3-phosphate dehydrogenase 1 , GPD-C , GPDH-C , HTGTI

Cellular Localization

Cytoplasm

Database

Research Area

DATA IMAGES

Anti-GPD1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117910

GTX117910 ICC/IF Image

GPD1 antibody detects GPD1 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GPD1 protein stained by GPD1 antibody (GTX117910) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-GPD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117910

GTX117910 IHC-P Image

GPD1 antibody detects GPD1 protein at cytosol on D54MG xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded D54MG xenograft.
GPD1 antibody (GTX117910) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GPD1 antibody used in Western Blot (WB). GTX117910

GTX117910 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX117910 diluted at 1:1000

Anti-GPD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117910

GTX117910 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using GPD1(GTX117910) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GPD1 antibody used in IHC (Paraffin sections) (IHC-P). GTX117910

GTX117910 IHC-P Image

GPD1 antibody detects GPD1 protein at cytosol on Mahlarvu xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Mahlarvu xenograft.
GPD1 antibody (GTX117910) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169