GPD2 antibody [N1N3]

Cat. No. GTX114447

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all GPD2 products

Datasheet
Document

View product citations for antibody GTX114447 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000

Not tested in other applications.

Calculated MW

81 kDa. ( Note )

Positive Control

SK-N-SH

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.56 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GPD2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174517

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycerol-3-phosphate dehydrogenase 2 , GDH2 , GPDM , mGPDH

Cellular Localization

Mitochondrion

Background

Mitochondrial glycerophosphate dehydrogenase (EC 1.1.99.5), or GPD2, is located on the outer surface of the inner mitochondrial membrane and catalyzes the unidirectional conversion of glycerol-3-phosphate (G-3-P) to dihydroxyacetone phosphate (DHAP) with concomitant reduction of the enzyme-bound FAD. Together with a cytosolic NAD-linked GPD (GPD1; MIM 138420), GPD2 forms the glycerol phosphate shuttle, which uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, resulting in the reoxidation of NADH formed during glycolysis.[supplied by OMIM]

Database

Gene ID: 2820 GPD2
UniProt: P43304 GPD2

Research Area

DATA IMAGES

GTX114447 WB Image

Sample (30 ug of whole cell lysate)
A: SK-N-SH
10% SDS PAGE
GTX114447 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty