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GPI antibody [5A11]

KO/KD Overexpression
Anti-GPI antibody [5A11] used in Western Blot (WB). GTX84416
Anti-GPI antibody [5A11] used in Flow cytometry (FACS). GTX84416
Anti-GPI antibody [5A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84416
Anti-GPI antibody [5A11] used in Western Blot (WB). GTX84416
Anti-GPI antibody [5A11] used in IHC (Paraffin sections) (IHC-P). GTX84416
Anti-GPI antibody [5A11] used in IHC (Paraffin sections) (IHC-P). GTX84416
Anti-GPI antibody [5A11] used in Western Blot (WB). GTX84416

Cat. No. GTX84416

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5A11

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Dog, Monkey
Package
100 μl ($519)
See all GPI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human GPI(NP_000166) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glucose-6-phosphate isomerase , AMF , GNPI , NLK , PGI , PHI , SA-36 , SA36

Cellular Localization

Cytoplasm,Secreted

Background

Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.

Database

Research Area

DATA IMAGES

Anti-GPI antibody [5A11] used in Western Blot (WB). GTX84416

GTX84416 WB Image

Overexpression

WB analysis of HEK293T cells transfected with GPI plasmid (Right) or empty vector (Left) for 48 hrs using GTX84416 GPI antibody [5A11].
Loading : 5 ug per lane

Anti-GPI antibody [5A11] used in Flow cytometry (FACS). GTX84416

GTX84416 FACS Image

FACS analysis of Jurkat cells using GTX84416 GPI antibody [5A11].
Red : Primary antibody
Blue : Negative control antibody

Anti-GPI antibody [5A11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84416

GTX84416 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with GPI plasmid using GTX84416 GPI antibody [5A11].

Anti-GPI antibody [5A11] used in Western Blot (WB). GTX84416

GTX84416 WB Image

KO/KD

WB analysis of wild type and GPI knock out 293T cell lysate(10μg per lane) using GTX84416 GPI antibody [5A11].
Dilution : 1:500

Anti-GPI antibody [5A11] used in IHC (Paraffin sections) (IHC-P). GTX84416

GTX84416 IHC-P Image

IHC-P analysis of human liver tissue using GTX84416 GPI antibody [5A11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-GPI antibody [5A11] used in IHC (Paraffin sections) (IHC-P). GTX84416

GTX84416 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX84416 GPI antibody [5A11].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-GPI antibody [5A11] used in Western Blot (WB). GTX84416

GTX84416 WB Image

WB analysis of various cell lines using GTX84416 GPI antibody [5A11].
Loading : 35 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519