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GPI antibody [7G10]

KO/KD Overexpression
Anti-GPI antibody [7G10] used in IHC (Paraffin sections) (IHC-P). GTX84417
Anti-GPI antibody [7G10] used in IHC (Paraffin sections) (IHC-P). GTX84417
Anti-GPI antibody [7G10] used in Western Blot (WB). GTX84417
Anti-GPI antibody [7G10] used in Western Blot (WB). GTX84417
Anti-GPI antibody [7G10] used in IHC (Paraffin sections) (IHC-P). GTX84417
Anti-GPI antibody [7G10] used in IHC (Paraffin sections) (IHC-P). GTX84417

Cat. No. GTX84417

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7G10

Isotype

 IgG2a

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($519)
See all GPI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
IHC-P 1:50
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human GPI(NP_000166) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glucose-6-phosphate isomerase , AMF , GNPI , NLK , PGI , PHI , SA-36 , SA36

Cellular Localization

Cytoplasm,Secreted

Background

Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.

Database

Research Area

DATA IMAGES

Anti-GPI antibody [7G10] used in IHC (Paraffin sections) (IHC-P). GTX84417

GTX84417 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84417 GPI antibody [7G10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-GPI antibody [7G10] used in IHC (Paraffin sections) (IHC-P). GTX84417

GTX84417 IHC-P Image

IHC-P analysis of kidney tissue using GTX84417 GPI antibody [7G10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-GPI antibody [7G10] used in Western Blot (WB). GTX84417

GTX84417 WB Image

KO/KD

WB analysis of wild type and GPI knock out 293T cell lysate(10μg per lane) using GTX84417 GPI antibody [7G10].
Dilution : 1:500

Anti-GPI antibody [7G10] used in Western Blot (WB). GTX84417

GTX84417 WB Image

Overexpression

WB analysis of HEK293T cells transfected with GPI plasmid (Right) or empty vector (Left) for 48 hrs using GTX84417 GPI antibody [7G10].
Loading : 5 ug per lane

Anti-GPI antibody [7G10] used in IHC (Paraffin sections) (IHC-P). GTX84417

GTX84417 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84417 GPI antibody [7G10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-GPI antibody [7G10] used in IHC (Paraffin sections) (IHC-P). GTX84417

GTX84417 IHC-P Image

IHC-P analysis of liver tissue using GTX84417 GPI antibody [7G10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519