GeneTex
United States (US)

GPI antibody, Internal (near N-term)

GTX88240_2446_WB_18122102_270.jpg
GTX88240_1020_IHC-P_18122102_735.jpg
GTX88240_1019_IHC-P_18122102_692.jpg
GTX88240_1021_IHC-P_18122102_467.jpg
GTX88240_2445_WB_18122102_919.jpg

Cat No. GTX88240

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88240-PEP).
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-RERMFNGEKINYT, from the internal region (near N Terminus) of the protein sequence according to NP_000166.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

PHI Antibody , AMF Antibody , NLK Antibody , GNPI Antibody , PGI Antibody , SA36 Antibody

Background

This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
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Database

Research Area

DATA IMAGES
GTX88240_2446_WB_18122102_270.jpg

GTX88240 WB Image

WB analysis of human heart lysate using GTX88240 GPI antibody, Internal (near N-term).
Dilution : 0.3µg/ml
Loading : 35µg protein in RIPA buffer

GTX88240_1020_IHC-P_18122102_735.jpg

GTX88240 IHC-P Image

IHC-P analysis of human testis using GTX88240 GPI antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88240_1019_IHC-P_18122102_692.jpg

GTX88240 IHC-P Image

IHC-P analysis of human kidney using GTX88240 GPI antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88240_1021_IHC-P_18122102_467.jpg

GTX88240 IHC-P Image

IHC-P analysis of human thyroid using GTX88240 GPI antibody, Internal (near N-term).
Antigen retrieval : citrate buffer Ph 6
Dilution : 3.75µg/ml

GTX88240_2445_WB_18122102_919.jpg

GTX88240 WB Image

WB analysis of HeLa (A), A431 (B), A549 (C), MCF7 (D), Jurkat (E) and K562 (F) lysates using GTX88240 GPI antibody, Internal (near N-term).
Dilution : 0.05µg/ml
Loading : 35µg protein in RIPA buffer