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GPI antibody, Internal (near N-term)

Anti-GPI antibody, Internal (near N-term) used in Western Blot (WB). GTX88240
Anti-GPI antibody, Internal (near N-term) used in IHC (Paraffin sections) (IHC-P). GTX88240
Anti-GPI antibody, Internal (near N-term) used in IHC (Paraffin sections) (IHC-P). GTX88240
Anti-GPI antibody, Internal (near N-term) used in IHC (Paraffin sections) (IHC-P). GTX88240
Anti-GPI antibody, Internal (near N-term) used in Western Blot (WB). GTX88240

Cat No. GTX88240

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

63 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88240-PEP).

PROPERTIES

Form

Liquid

Buffer

TBS pH7.3, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-RERMFNGEKINYT, from the internal region (near N Terminus) of the protein sequence according to NP_000166.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

glucose-6-phosphate isomerase , AMF , GNPI , NLK , PGI , PHI , SA-36 , SA36

Cellular Localization

Cytoplasm , Secreted

Background

This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]

Database

Research Area

DATA IMAGES

Anti-GPI antibody, Internal (near N-term) used in Western Blot (WB). GTX88240

GTX88240 WB Image

WB analysis of human heart lysate using GTX88240 GPI antibody, Internal (near N-term).
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

Anti-GPI antibody, Internal (near N-term) used in IHC (Paraffin sections) (IHC-P). GTX88240

GTX88240 IHC-P Image

IHC-P analysis of human testis using GTX88240 GPI antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

Anti-GPI antibody, Internal (near N-term) used in IHC (Paraffin sections) (IHC-P). GTX88240

GTX88240 IHC-P Image

IHC-P analysis of human kidney using GTX88240 GPI antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

Anti-GPI antibody, Internal (near N-term) used in IHC (Paraffin sections) (IHC-P). GTX88240

GTX88240 IHC-P Image

IHC-P analysis of human thyroid using GTX88240 GPI antibody, Internal (near N-term).
Antigen retrieval : citrate buffer Ph 6
Dilution : 3.75μg/ml

Anti-GPI antibody, Internal (near N-term) used in Western Blot (WB). GTX88240

GTX88240 WB Image

WB analysis of HeLa (A), A431 (B), A549 (C), MCF7 (D), Jurkat (E) and K562 (F) lysates using GTX88240 GPI antibody, Internal (near N-term).
Dilution : 0.05μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

There are currently no reviews for GPI antibody, Internal (near N-term) (GTX88240). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 379