APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.1-0.3μg/ml |
| 3.75μg/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX88240-PEP).
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-RERMFNGEKINYT, from the internal region (near N Terminus) of the protein sequence according to NP_000166.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
glucose-6-phosphate isomerase , AMF , GNPI , NLK , PGI , PHI , SA-36 , SA36
Cellular Localization
Cytoplasm , Secreted
Background
This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phosphate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2016]
Database
Research Area
DATA IMAGES
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GTX88240 WB Image
WB analysis of HeLa (A), A431 (B), A549 (C), MCF7 (D), Jurkat (E) and K562 (F) lysates using GTX88240 GPI antibody, Internal (near N-term).
Dilution : 0.05μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88240 IHC-P Image
IHC-P analysis of human thyroid using GTX88240 GPI antibody, Internal (near N-term).
Antigen retrieval : citrate buffer Ph 6
Dilution : 3.75μg/ml
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GTX88240 IHC-P Image
IHC-P analysis of human testis using GTX88240 GPI antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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GTX88240 WB Image
WB analysis of human heart lysate using GTX88240 GPI antibody, Internal (near N-term).
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer
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GTX88240 IHC-P Image
IHC-P analysis of human kidney using GTX88240 GPI antibody, Internal (near N-term).
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml
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REFERENCE
There are currently no references for GPI antibody, Internal (near N-term) (GTX88240). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for GPI antibody, Internal (near N-term) (GTX88240). Be the first to share your experience with this product.
