GeneTex
United States (US)

GPI antibody

Anti-GPI antibody used in Western Blot (WB). GTX33226
Anti-GPI antibody used in IHC (Paraffin sections) (IHC-P). GTX33226
Anti-GPI antibody used in IHC (Paraffin sections) (IHC-P). GTX33226

Cat No. GTX33226

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

63 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human GPI (NP_000166.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Glucose-6-Phosphate Isomerase,Amf,Gnpi,Nlk,Pgi,Phi,Sa-36,Sa36,Gpi

Background

This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Database

Research Area

DATA IMAGES
Anti-GPI antibody used in Western Blot (WB). GTX33226

GTX33226 WB Image

WB analysis of various samples using GTX33226 GPI antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-GPI antibody used in IHC (Paraffin sections) (IHC-P). GTX33226

GTX33226 IHC-P Image

IHC-P analysis of human prostate tissue using GTX33226 GPI antibody.
Dilution : 1:100

Anti-GPI antibody used in IHC (Paraffin sections) (IHC-P). GTX33226

GTX33226 IHC-P Image

IHC-P analysis of human lung tissue using GTX33226 GPI antibody.
Dilution : 1:100

Package List Price ($)
$ 329