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GPKOW antibody [N3C3]

Anti-GPKOW antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX117716
Anti-GPKOW antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX117716
Anti-GPKOW antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117716
Anti-GPKOW antibody [N3C3] used in Western Blot (WB). GTX117716

Cat. No. GTX117716

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all GPKOW products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

A549 , HeLa

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human GPKOW. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618017

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

G-patch domain and KOW motifs , GPATC5 , GPATCH5 , Spp2 , T54

Cellular Localization

Nucleus

Database

DATA IMAGES

Anti-GPKOW antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX117716

GTX117716 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SCM1 xenograft, using GPKOW(GTX117716) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-GPKOW antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX117716

GTX117716 IHC-P Image

GPKOW antibody [N3C3] detects GPKOW protein at nucleus in human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human breast cancer.
GPKOW antibody [N3C3] (GTX117716) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-GPKOW antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117716

GTX117716 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using GPKOW(GTX117716) antibody at 1:200 dilution.

Anti-GPKOW antibody [N3C3] used in Western Blot (WB). GTX117716

GTX117716 WB Image

Sample (30 ug of whole cell lysate)
A: A549
B: Hela
10% SDS PAGE
GTX117716 diluted at 1:1000

REFERENCE

Application Reference

Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : WB, ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169